Human C9orf69 knockout HeLa cell lysate
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TMEM250 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2 and 71 bp deletion in exon2.
View Alternative Names
C9orf69, CI069_HUMAN, Herpes virus UL25-binding protein, Protein C9orf69
- Sanger seq
Unknown
Sanger Sequencing - Human C9orf69 knockout HeLa cell lysate (AB263131)
Allele-2 : 1 bp deletion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human C9orf69 knockout HeLa cell lysate (AB263131)
Allele-1 : 71 bp deletion in exon2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
C9orf69 plays a role in cellular processes related to immune response and cell signaling. This protein is not yet associated with any specific complex but its presence in immune cells suggests an involvement in regulation or response mechanisms. Its functions contribute to the maintenance of cellular activities especially related to homeostasis and stress responses.
Pathways
Multiple cellular processes engage C9orf69. It associates with signaling pathways that mediate cellular responses including the immune response and intracellular signaling networks. C9orf69 shows functional connectivity to proteins such as NF-kB a critical regulator of immune and inflammatory responses indicating its potential role in modulating these pathways.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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