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AB273778

Human CA12 knockout A549 cell lysate

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CA12 KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9 X = 1 bp insertion Frameshift: 96%.

View Alternative Names

CA 12, CA-XII, CAH12_HUMAN, Carbonate dehydratase XII, Carbonic anhydrase 12, Carbonic anhydrase XII, Carbonic dehydratase, FLJ20151, HsT18816, T18816, Tumor antigen HOM-RCC-3.1.3

2 Images
Western blot - Human CA12 knockout A549 cell lysate (AB273778)
  • WB

Lab

Western blot - Human CA12 knockout A549 cell lysate (AB273778)

Lane 1 : Wild-type A549 (Human lung carcinoma cell line) whole cell lysate, 20 ug
Lane 2 : Ca12 knockout A549 (Human lung carcinoma cell line) whole cell lysate, 20 ug
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate, 20 ug

Lanes 1 - 3 : Merged signal (red and green). Green - ab195233 observed at 40 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.

ab195233 was shown to react with Carbonic Anhydrase 12/CA12 in wild-type A549 cells in Western blot with loss of signal observed in Ca12 knockout cell line ab273738 (knockout cell lysate ab273778). Wild-type A549 and Ca12 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab195233 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (<a href='/en-us/products/primary-antibodies/carbonic-anhydrase-12-ca12-antibody-epr14861-c-terminal-ab195233'>ab195233</a>) at 1/10000 dilution

Lane 1:

Wild-type A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

Ca12 knockout A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

Western blot - Human CA12 knockout A549 cell line (<a href='/en-us/products/cell-lines/human-ca12-knockout-a549-cell-line-ab273738'>ab273738</a>)

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Predicted band size: 39 kDa

Observed band size: 40 kDa

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Next Generation Sequencing - Human CA12 knockout A549 cell lysate (AB273778)
  • NGS

Supplier Data

Next Generation Sequencing - Human CA12 knockout A549 cell lysate (AB273778)

Knockout achieved by CRISPR/Cas9; X = 1 bp insertion; Frameshift : 96%

Key facts

Cell type

A549

Species or organism

Human

Tissue

Lung

Knockout validation

Next Generation Sequencing,Western blot

Mutation description

Knockout achieved by CRISPR/Cas9 X = 1 bp insertion Frameshift: 96%

Disease

Carcinoma

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
CA12
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Next Generation Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Male

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Product protocols

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Complementary Products

Primary Antibodies

AB195233

Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal

RabMAb|Recombinant|KO Validated

Western blot - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (AB195233)

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Cell Lines & Lysates

AB273738

Human CA12 knockout A549 cell line

Advanced Validation

Western blot - Human CA12 knockout A549 cell line (AB273738)

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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