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AB257084

Human CA2 (Carbonic anhydrase 2) knockout HEK-293T cell lysate

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CA2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.

View Alternative Names

CA 2, CA-II, CAC, CAH2_HUMAN, Car 2, Carbonate dehydratase II, Carbonic anhydrase 2, Carbonic anhydrase B, Carbonic anhydrase C, Carbonic anhydrase C, formerly, Carbonic anhydrase II, Carbonic dehydratase, Epididymis secretory protein Li 282, HEL-76, HEL-S-282, epididymis luminal protein 76

3 Images
Western blot - Human CA2 (Carbonic anhydrase 2) knockout HEK-293T cell lysate (AB257084)
  • WB

Unknown

Western blot - Human CA2 (Carbonic anhydrase 2) knockout HEK-293T cell lysate (AB257084)

Lane 1 : Wild-type HEK293T cell lysate (20 ug)
Lane 2 : CA2 knockout HEK293T cell lysate (20 ug)
Lane 3 : A431 cell lysate (20 ug)
Lane 4 : Caco-2 cell lysate (20 ug)

ab82559 was shown to specifically react with Carbonic anhydrase 2/CA2 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab265072 (knockout cell lysate ab257084) was used. Wild-type and Carbonic anhydrase 2/CA2 knockout samples were subjected to SDS-PAGE. ab82559 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Anti-Carbonic anhydrase 2/CA2 antibody (<a href='/en-us/products/unavailable/carbonic-anhydrase-2ca2-antibody-ab82559'>ab82559</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

CA2 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human CA2 (Carbonic anhydrase 2) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-ca2-carbonic-anhydrase-2-knockout-hek-293t-cell-line-ab265072'>ab265072</a>)

Lane 3:

A431 cell lysate at 20 µg

Lane 4:

Caco-2 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

false

Western blot - Human CA2 (Carbonic anhydrase 2) knockout HEK-293T cell lysate (AB257084)
  • WB

Lab

Western blot - Human CA2 (Carbonic anhydrase 2) knockout HEK-293T cell lysate (AB257084)

Lane 1 : Wild-type HEK293T cell lysate (20 ug)
Lane 2 : CA2 knockout HEK293T cell lysate (20 ug)
Lane 3 : A431 cell lysate (20 ug)
Lane 4 : Caco-2 cell lysate (20 ug)

ab124687 was shown to specifically react with Carbonic anhydrase 2/CA2 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab265072 (knockout cell lysate ab257084) was used. Wild-type and Carbonic anhydrase 2/CA2 knockout samples were subjected to SDS-PAGE. ab124687 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Carbonic anhydrase 2/CA2 antibody [EPR5195] (<a href='/en-us/products/primary-antibodies/carbonic-anhydrase-2-ca2-antibody-epr5195-ab124687'>ab124687</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

CA2 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human CA2 (Carbonic anhydrase 2) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-ca2-carbonic-anhydrase-2-knockout-hek-293t-cell-line-ab265072'>ab265072</a>)

Lane 3:

A431 cell lysate at 20 µg

Lane 4:

Caco-2 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

Sanger Sequencing - Human CA2 (Carbonic anhydrase 2) knockout HEK-293T cell lysate (AB257084)
  • Sanger seq

Unknown

Sanger Sequencing - Human CA2 (Carbonic anhydrase 2) knockout HEK-293T cell lysate (AB257084)

Homozygous : 1 bp insertion in exon 1

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab257084-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human wild-type HEK293T cell lysate", "number":"AB257084-CMP02", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human CA2 knockout HEK293T cell lysate", "number":"AB257084-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Gene name
CA2
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Zygosity
Homozygous
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Complementary Products

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AB124687

Anti-Carbonic anhydrase 2/CA2 antibody [EPR5195]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic anhydrase 2/CA2 antibody [EPR5195] (AB124687)

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