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AB257374

Human CACNA2D1 (Calcium channel L type DHPR alpha 2 subunit) knockout HeLa cell lysate

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CACNA2D1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon11 and 14 bp deletion in exon11 and 1 bp insertion in exon11.
4 Images
Western blot - Human CACNA2D1 (Calcium channel L type DHPR alpha 2 subunit) knockout HeLa cell lysate (AB257374)
  • WB

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Western blot - Human CACNA2D1 (Calcium channel L type DHPR alpha 2 subunit) knockout HeLa cell lysate (AB257374)

Lane 1 : Wild-type HeLa cell lysate (20 μg)

Lane 2 : CACNA2D1 knockout HeLa cell lysate (20 μg)

Lanes 1-2 : Merged signal (red and green). Green - ab2864 observed at 150 kDa. Red - loading control ab181602 observed at 37 kDa.

ab2864 Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [20A] was shown to specifically react with Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265080 (knockout cell lysate ab257374) was used. Wild-type and Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 knockout samples were subjected to SDS-PAGE. ab2864 and Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) were incubated overnight at 4°CC at 1 in 500 Dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [20A] (<a href='/en-us/products/unavailable/calcium-channel-l-type-dhpr-alpha-2-subunitcacna2d1-antibody-20a-ab2864'>ab2864</a>) at 1/500 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

CACNA2D1 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human CACNA2D1 (Calcium channel L type DHPR alpha 2 subunit) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-cacna2d1-calcium-channel-l-type-dhpr-alpha-2-subunit-knockout-hela-cell-line-ab265080'>ab265080</a>)

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Sanger Sequencing - Human CACNA2D1 (Calcium channel L type DHPR alpha 2 subunit) knockout HeLa cell lysate (AB257374)
  • Sanger seq

Unknown

Sanger Sequencing - Human CACNA2D1 (Calcium channel L type DHPR alpha 2 subunit) knockout HeLa cell lysate (AB257374)

Allele-3 : 1 bp insertion in exon11

Sanger Sequencing - Human CACNA2D1 (Calcium channel L type DHPR alpha 2 subunit) knockout HeLa cell lysate (AB257374)
  • Sanger seq

Unknown

Sanger Sequencing - Human CACNA2D1 (Calcium channel L type DHPR alpha 2 subunit) knockout HeLa cell lysate (AB257374)

Allele-2 : 11 bp deletion in exon11

Sanger Sequencing - Human CACNA2D1 (Calcium channel L type DHPR alpha 2 subunit) knockout HeLa cell lysate (AB257374)
  • Sanger seq

Lab

Sanger Sequencing - Human CACNA2D1 (Calcium channel L type DHPR alpha 2 subunit) knockout HeLa cell lysate (AB257374)

Allele-1 : 14 bp deletion in exon11

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon11 and 14 bp deletion in exon11 and 1 bp insertion in exon11.

Disease

Adenocarcinoma

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Complementary Products

Primary Antibodies

AB253190

Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-8]

RabMAb|Recombinant|Trial Size|Trial Size

Western blot - Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-8] (AB253190)

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Proteins & Peptides

AB259412

Recombinant human VEGF 165A protein (Active)

Premium Bioactive

Mass Spectrometry - Recombinant human VEGF 165A protein (Active) (AB259412)

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Proteins & Peptides

AB180317

Recombinant Human POLR1C protein

SDS-PAGE - Recombinant Human POLR1C protein (AB180317)

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Biochemicals

AB120219

Gabapentin, alpha2delta Ligand

Chemical Structure - Gabapentin, alpha2delta Ligand (AB120219)

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  • 0
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Reactivity data

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
CACNA2D1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Calcium channel L type DHPR alpha 2 subunit also known as CACNA2D1 or alpha-2-delta plays a significant role in cellular calcium regulation. This subunit typically part of the voltage-gated calcium channel complex allows calcium ions to enter the cells following electrical stimuli. With a mass of about 150 kDa CACNA2D1 expresses predominantly in skeletal and cardiac muscle tissues and the central nervous system. Its presence across different tissues suggests various physiological roles linking its mechanical function to electrical signaling and muscle contraction.
Biological function summary

CACNA2D1 aids in modulating neurotransmitter release and excitation-contraction coupling. This protein often integrates into the larger voltage-gated calcium channel complex working in concert with other subunits to optimize calcium flow. This particular function supports critical processes in muscle cells and neurons integrating signals in response to electrical impulses. The alpha-2-delta ligand poses significance here as well often used to modify the channel's activity and study its role in cellular communication.

Pathways

CACNA2D1 actively participates in electrical signaling and muscle contraction pathways. It contributes to the excitation-contraction coupling pathway in muscle cells aligning closely with proteins like the ryanodine receptor ensuring efficient calcium-induced calcium release. Its action also extends to the neurotransmitter release pathway where it associates with synaptic proteins regulating neurotransmission. The function within these pathways underlines its importance in both muscle and neural activities.

CACNA2D1 associates with conditions such as epilepsy and neuropathic pain. The dysfunction in its channel activity can lead to altered calcium flow contributing to these disorders. The alpha-2-delta ligand such as the gabapentin chemical structure plays a role here often targeting the channel to mitigate symptoms. Gabapentin's influence highlights the therapeutic potential of targeting CACNA2D1 in disease management providing critical insights into neuromodulation treatments.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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