AB257375

Human CALD1 (Caldesmon/CDM) knockout HeLa cell lysate

Name: Human CALD1 (Caldesmon/CDM) knockout HeLa cell lysate (ab257375) | Abcam
Brand: Abcam Limited
SKU: AB257375
Price: 670 USD
Availability: InStock

Be the first to review this product! Submit a review

(0 Publication)

CALD1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.

View Alternative Names

CAD, CDM, Caldesmon, CALD1

3 Images

Lab

Western blot - Human CALD1 (Caldesmon/CDM) knockout HeLa cell lysate (AB257375)

Lane 1 : Wild-type HeLa cell lysate (20 μg)

Lane 2 : CALD1 knockout HeLa cell lysate (20 μg)

Lane 3 : NIH/3T3 cell lysate (20 μg)

Lane 4 : HEK-293 cell lysate (20 μg)

Lanes 1-4 : Merged signal (red and green). Green - ab183339 observed at 75 kDa. Red - loading control ab8245 observed at 36 kDa.

ab183339 Anti-Caldesmon/CDM antibody [SP226] was shown to specifically react with Caldesmon/CDM in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265026 (knockout cell lysate ab257375) was used. Wild-type and Caldesmon/CDM knockout samples were subjected to SDS-PAGE. ab183339 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Caldesmon/CDM antibody [SP226] (<a href='/en-us/products/primary-antibodies/caldesmon-cdm-antibody-sp226-ab183339'>ab183339</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

CALD1 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human CALD1 (Caldesmon/CDM) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-cald1-caldesmon-cdm-knockout-hela-cell-line-ab265026'>ab265026</a>)

Lane 3:

NIH/3T3 cell lysate at 20 µg

Lane 4:

HEK-293 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 93 kDa

Observed band size: 75 kDa

false

Lab

Western blot - Human CALD1 (Caldesmon/CDM) knockout HeLa cell lysate (AB257375)

Lane 1 : Wild-type HeLa cell lysate (20 μg)

Lane 2 : CALD1 knockout HeLa cell lysate (20 μg)

Lane 3 : NIH/3T3 cell lysate (20 μg)

Lane 4 : HEK-293 cell lysate (20 μg)

Lanes 1-4 : Merged signal (red and green). Green - ab32330 observed at 75 kDa. Red - loading control ab8245 observed at 36 kDa.

ab32330 Anti-Caldesmon/CDM antibody [E89] was shown to specifically react with Caldesmon/CDM in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265026 (knockout cell lysate ab257375) was used. Wild-type and Caldesmon/CDM knockout samples were subjected to SDS-PAGE. ab32330 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Caldesmon/CDM antibody [E89] (<a href='/en-us/products/primary-antibodies/caldesmon-cdm-antibody-e89-ab32330'>ab32330</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

CALD1 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human CALD1 (Caldesmon/CDM) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-cald1-caldesmon-cdm-knockout-hela-cell-line-ab265026'>ab265026</a>)

Lane 3:

NIH/3T3 cell lysate at 20 µg

Lane 4:

HEK-293 cell lysate at 20 µg

Secondary

All lanes:

Predicted band size: 93 kDa

Observed band size: 75 kDa

false

Sanger Sequencing - Human CALD1 (Caldesmon/CDM) knockout HeLa cell lysate (AB257375)

Sanger seq

Unknown

Sanger Sequencing - Human CALD1 (Caldesmon/CDM) knockout HeLa cell lysate (AB257375)

Homozygous : 1 bp insertion in exon 1

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.

Disease

Adenocarcinoma

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

style

left-column

What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab257375-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human CALD1 knockout HeLa cell lysate", "number":"AB257375-CMP02", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HeLa cell lysate", "number":"AB257375-CMP01", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Gene name

CALD1

Gene editing type

Knockout

Gene editing method

CRISPR technology

Knockout validation

Sanger Sequencing, Western blot

Zygosity

Homozygous

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Caldesmon also known as CDM CALD1 and h-caldesmon is a cytoskeletal protein with a molecular mass of approximately 93 to 120 kDa. This protein expresses itself abundantly in smooth muscle tissues yet it can also be found in non-muscle cells like fibroblasts and endothelial cells. Researchers often use specific caldesmon antibodies in immunohistochemistry allowing them to label various cellular components and providing insights into tissue composition. Due to its presence in muscle tissues caldesmon is essential for understanding muscle physiology and pathology.

Biological function summary

Caldesmon interacts with actin and myosin to regulate actomyosin contractility. This protein plays a critical role in controlling the contraction and relaxation processes in smooth muscle cells. Caldesmon forms part of a complex that includes calmodulin and tropomyosin enhancing its ability to stabilize actin filaments. It functions by inhibiting the ATPase activity of myosin therefore influencing cellular motility and shape change mechanisms. Researchers continually study caldesmon to comprehend its interactome and its significance within the larger cellular structure.

Pathways

Caldesmon participates in the regulation of the cytoskeletal dynamics vital for cell motility and structural integrity. In particular it is an important component of the contraction-relaxation cycle pathway in smooth muscle tissues. This protein has connections with pathways involving RhoA-Rho kinase where caldesmon modulates the phosphorylation levels influencing muscle contraction. Additionally proteins like tropomyosin and calmodulin modulate its activity especially under calcium-calmodulin-dependent pathways which further elucidates its regulatory importance.

Caldesmon has associations with conditions like certain types of cancers and cardiovascular diseases. Its expression levels and distribution provide valuable information in identifying smooth muscle tumors and other pathological conditions. In oncology for example h-caldesmon serves as a marker to distinguish leiomyosarcomas from other tumors. Moreover due to its involvement in smooth muscle contractility caldesmon links with proteins such as calmodulin and tropomyosin in diseases where abnormal contraction and cellular motility play significant roles. Understanding these connections is important for developing targeted treatments and improving diagnostic accuracy.

style

left-column

Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

style

left-column

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

style

left-column

Product protocols

Visit the General protocols
Visit the Troubleshooting

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human CALD1 (Caldesmon/CDM) knockout HeLa cell lysate

Western blot - Human CALD1 (Caldesmon/CDM) knockout HeLa cell lysate (AB257375)

Western blot - Human CALD1 (Caldesmon/CDM) knockout HeLa cell lysate (AB257375)

Sanger Sequencing - Human CALD1 (Caldesmon/CDM) knockout HeLa cell lysate (AB257375)

Key facts

Cell type

Species or organism

Tissue

Knockout validation

Mutation description

Disease

Complementary Products

Primary Antibodies

Proteins & Peptides

Proteins & Peptides

Primary Antibodies

Reactivity data

Product details

What's included?

Properties and storage information

Gene name

Gene editing type

Gene editing method

Knockout validation

Zygosity

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Quality control

STR analysis

Cell culture

Biosafety level

Adherent/suspension

Gender

Product protocols

Product promise