Human CALM2 knockout HeLa cell lysate
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CALM2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 271 bp deletion in exon 2.
View Alternative Names
CAM, CAM 2, CAMB, CAMII, Calmodulin 2 (Phosphorylase Kinase, Delta), Calmodulin-2, LP7057 Protein, LQT15, PHKD, PHKD2, Phosphorylase Kinase Delta, Phosphorylase Kinase Subunit Delta, Prepro-Calmodulin 2
- Sanger seq
Unknown
Sanger Sequencing - Human CALM2 knockout HeLa cell lysate (AB263134)
Homozygous : 271 bp deletion in exon2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CALM2 acts as a critical mediator in calcium signaling pathways. It forms complexes with other proteins to transmit calcium signals that are important for activities such as muscle contraction cell division and memory formation. CALM2 activates various enzymes ion channels and other proteins through its calcium-binding ability thereby translating calcium ion fluctuations into cellular responses.
Pathways
The involvement of CALM2 in calcium-dependent pathways like the calcium signaling pathway and the MAPK/ERK pathway is significant. It interacts with proteins such as calcineurin and kinases like calcium/calmodulin-dependent protein kinase (CaMK) facilitating the transmission of signals inside cells. Its role in these pathways highlights its importance in regulating diverse cellular activities including cell proliferation and gene expression.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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