Human CALM3 knockout HEK-293T cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human CALM3 knockout HEK-293T cell lysate (AB263135)
Allele-2 : 1 bp insertion in exon 3
- Sanger seq
Unknown
Sanger Sequencing - Human CALM3 knockout HEK-293T cell lysate (AB263135)
Allele-1 : 20 bp deletion in exon 3
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Beyond its role in calcium binding CALM3 interacts with a wide network of proteins forming complexes that regulate cellular activities. It modulates enzyme activities and ion channels influencing cellular signaling pathways. Its central position allows it to impact diverse biological functions such as muscle contraction intracellular transport and cell division. It operates intricately with its family members CALM1 and CALM2 showing how calmodulin proteins often work collectively in cellular environments.
Pathways
CALM3 significantly influences the calcium signaling pathway and plays a critical role in the phosphoinositide pathway. In calcium signaling it helps in translating calcium fluctuations into specific cellular responses interacting with proteins like CaMKII and calcineurin which are pivotal in maintaining cellular homeostasis. The involvement in the phosphoinositide pathway further links it with membrane trafficking and signal transduction demonstrating its connectivity within cellular signaling networks.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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