CAMK2G KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon4 and 1 bp insertion in exon4 and 2 bp deletion in exon4.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Sanger Sequencing, Western blot
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon4 and 1 bp insertion in exon4 and 2 bp deletion in exon4.
Alternative names
CAMK, CAMK II, CAMK2G, CAMKG, CaM kinase II, CaM kinase II gamma chain, CaM kinase II subunit gamma, CaMK II subunit gamma
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CAMK2G KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon4 and 1 bp insertion in exon4 and 2 bp deletion in exon4.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Sanger Sequencing, Western blot
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon4 and 1 bp insertion in exon4 and 2 bp deletion in exon4.
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- CAMK2G
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing, Western blot
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Western blot - Human CAMK2G (CamKII gamma) knockout HeLa cell lysate (ab258340)
Lane 1: Wild-type HeLa cell lysate (20 μg)Lane 2: CAMK2G knockout HeLa cell lysate (20 μg)
Lane 3: T-47D cell lysate (20 μg)
Lanes 1-3: Merged signal (red and green). Green - Anti-CamKII gamma antibody [8G10C1] ab201966 observed at 63 kDa. Red - loading control, Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 observed at 37 kDa.
Anti-CamKII gamma antibody [8G10C1] ab201966 Anti-CamKII gamma antibody [8G10C1] was shown to specifically react with CamKII gamma in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human CAMK2G (CamKII gamma) knockout HeLa cell line ab264712 (knockout cell lysate ab258340) was used. Wild-type and CamKII gamma knockout samples were subjected to SDS-PAGE. Anti-CamKII gamma antibody [8G10C1] ab201966 and Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-CamKII gamma antibody [8G10C1] (Anti-CamKII gamma antibody [8G10C1] ab201966) at 1/500 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: CAMK2G knockout HeLa cell lysate at 20 µg
Lane 3: T-47D cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 63 kDa
Observed band size: 63 kDa
Sanger Sequencing - Human CAMK2G (CamKII gamma) knockout HeLa cell lysate (ab258340)
Allele-2: 1 bp deletion in exon4
Sanger Sequencing - Human CAMK2G (CamKII gamma) knockout HeLa cell lysate (ab258340)
Allele-1: 2 bp deletion in exon4
Sanger Sequencing - Human CAMK2G (CamKII gamma) knockout HeLa cell lysate (ab258340)
Allele-3: 1 bp insertion in exon4
Downloads
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- Visit the general protocols
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