Human CAMK2G (CamKII gamma) knockout HeLa cell lysate
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(0 Publication)
- WB
Unknown
Western blot - Human CAMK2G (CamKII gamma) knockout HeLa cell lysate (AB258340)
Lane 1 : Wild-type HeLa cell lysate (20 μg)
Lane 2 : CAMK2G knockout HeLa cell lysate (20 μg)
Lane 3 : T-47D cell lysate (20 μg)
Lanes 1-3 : Merged signal (red and green). Green - ab201966 observed at 63 kDa. Red - loading control, ab181602 observed at 37 kDa.
ab201966 Anti-CamKII gamma antibody [8G10C1] was shown to specifically react with CamKII gamma in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264712 (knockout cell lysate ab258340) was used. Wild-type and CamKII gamma knockout samples were subjected to SDS-PAGE. ab201966 and Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-CamKII gamma antibody [8G10C1] (<a href='/en-us/products/primary-antibodies/camkii-gamma-antibody-8g10c1-ab201966'>ab201966</a>) at 1/500 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
CAMK2G knockout HeLa cell lysate at 20 µg
Lane 3:
T-47D cell lysate at 20 µg
Predicted band size: 63 kDa
Observed band size: 63 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human CAMK2G (CamKII gamma) knockout HeLa cell lysate (AB258340)
Allele-2 : 1 bp deletion in exon4
- Sanger seq
Unknown
Sanger Sequencing - Human CAMK2G (CamKII gamma) knockout HeLa cell lysate (AB258340)
Allele-1 : 2 bp deletion in exon4
- Sanger seq
Unknown
Sanger Sequencing - Human CAMK2G (CamKII gamma) knockout HeLa cell lysate (AB258340)
Allele-3 : 1 bp insertion in exon4
Complementary Products
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CamKII gamma antibody [8G10C1] (AB201966)](https://content.abcam.com/products/images/camkii-gamma-antibody-8g10c1-ab201966--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img131866.gif)
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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