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AB263136

Human CAMKK1 (CaMKK) knockout HeLa cell lysate

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CAMKK1 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2.
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Sanger Sequencing - Human CAMKK1 (CaMKK) knockout HeLa cell lysate (AB263136)
  • Sanger seq

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Sanger Sequencing - Human CAMKK1 (CaMKK) knockout HeLa cell lysate (AB263136)

Homozygous : 1 bp deletion in exon2

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2.

Disease

Adenocarcinoma

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Complementary Products

Cell Lines & Lysates

AB265635

Human CAMKK1 (CaMKK) knockout HeLa cell line

Sanger Sequencing - Human CAMKK1 (CaMKK) knockout HeLa cell line (AB265635)

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
CAMKK1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calcium/calmodulin-dependent protein kinase kinase (CaMKK) also known as CaMKKα and CaMKKβ depending on its isoform is an enzyme with a significant role in cellular signaling. It is a serine/threonine-specific protein kinase with a molecular mass of approximately 51–699 kDa depending on the isoform and expression variant. CaMKK is expressed in various tissues but shows high levels in the brain where it influences numerous neural processes. The enzyme works by phosphorylating and activating other kinases such as CaMK1 and CaMKIV thereby regulating a wide range of cellular activities.
Biological function summary

Calcium/calmodulin-dependent kinase kinase participates in calcium-mediated signal transduction pathways. This enzyme is part of a complex with calmodulin (CaM) a calcium-binding messenger protein which modulates its activity in response to intracellular calcium levels. CaMKK acts as a multifunctional kinase that can regulate neuronal functions energy balance and other critical cellular mechanisms.

Pathways

Calcium signaling and AMP-activated protein kinase (AMPK) pathways involve CaMKK. In calcium signaling CaMKK regulates CaMK series kinases which play roles in memory formation and synaptic plasticity. In the AMPK pathway CaMKKβ can activate AMPK a critical regulator of cellular energy homeostasis. CaMKK connects with proteins like CaMK1 CaMKIV and AMPK through these signaling systems helping maintain cellular responses to environmental and metabolic changes.

Calcium/calmodulin-dependent kinase kinase has associations with neurological and metabolic disorders. It influences conditions like Alzheimer's disease through its role in synaptic function and plasticity. Additionally dysregulation of CaMKKβ activity might be connected to obesity as it affects energy balance and glucose metabolism. In these disorders CaMKK interacts with proteins such as CaMKIV in Alzheimer’s influencing cognitive decline and AMPK in obesity impacting metabolic processes.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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