Human CAMKK2 knockout HEK-293T cell lysate
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- WB
Lab
Western blot - Human CAMKK2 knockout HEK-293T cell lysate (AB258341)
Lane 1 : Wild-type HEK-293T cell lysate 20 μg
Lane 2 : CAMKK2 knockout HEK-293T cell lysate 20 μg
Lane 3 : HeLa cell lysate 20 μg
False colour image of Western blot : Anti-CAMKK2 antibody staining at 1/500 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab96531 was shown to bind specifically to CAMKK2. A band was observed at 65 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in CAMKK2 knockout cell line ab266300 (knockout cell lysate ab258341). To generate this image, wild-type and CAMKK2 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
false
- Sanger seq
Unknown
Sanger Sequencing - Human CAMKK2 knockout HEK-293T cell lysate (AB258341)
Allele-1 : 10 bp deletion in exon 2
- Sanger seq
Unknown
Sanger Sequencing - Human CAMKK2 knockout HEK-293T cell lysate (AB258341)
Allele-2 : 1 bp insertion in exon 2
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Calcium/calmodulin-dependent protein kinase kinase 2 influences neuronal function through its regulation of calcium signaling pathways. CAMKK2 participates in the formation of a complex with calmodulin when calcium levels rise. This interaction modulates neurotransmitter release neuronal growth and synaptic plasticity. Beyond the nervous system CAMKK2 contributes to metabolic processes in the liver and insulin secretion in the pancreas thereby affecting whole-body energy homeostasis.
Pathways
Calcium/calmodulin-dependent protein kinase kinase 2 is involved in the calcium signaling and AMP-activated protein kinase (AMPK) pathways. In these pathways CAMKK2 interacts with CAMK1 CAMK4 and AMPK influencing cellular activities like energy balance and apoptosis. The activation of AMPK by CAMKK2 plays a significant role in maintaining cellular energy levels by regulating glucose and lipid metabolism linking it to diverse physiological functions.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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