Human CASC4 knockout HeLa cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human CASC4 knockout HeLa cell lysate (AB258802)
Allele-1 : 1 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human CASC4 knockout HeLa cell lysate (AB258802)
Allele-2 : 1 bp insertion in exon1
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The protein plays a role in cellular growth and regulation. It is part of a larger complex that includes other signaling and structural proteins supporting its function in maintaining proper cellular environments. It may help ensure the cell cycle progresses smoothly influencing cell survival and replication. Its expression suggests it might help modulate responses under stress or during abnormal growth which can have implications for understanding certain cancer behaviors.
Pathways
CASC4 engages in cellular signaling and growth regulation pathways specifically those related to cancer biology. It is involved in pathways such as the PI3K/AKT pathway which is important for cell proliferation and survival. In these networks CASC4 connects with other proteins like AKT potentially influencing the pathway’s outcomes on cell fate decisions and tumor progression. This interaction suggests that CASC4 may have a role in modifying signaling outcomes depending on different cellular contexts.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com