Human CASP1 (Caspase-1) knockout THP-1 cell lysate

• WB

Lab

Western blot - Human CASP1 (Caspase-1) knockout THP-1 cell lysate (AB277988)

Lane 1 : Wild-type THP-1 cell lysate 40 μg
Lane 2 : CASP1 knockout THP-1 cell lysate 40 μg
Lane 3 : HeLa cell lysate 20 μg
Lane 4 : HEK-293 cell lysate 20 μg
False colour image of Western blot : Anti-Caspase-1 antibody [EPR19672] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab207802 was shown to bind specifically to Caspase-1. A band was observed at 22, 35-40, 48 kDa in wild-type THP-1 cell lysates with no signal observed at this size in CASP1 knockout cell line ab276116 (knockout cell lysate ab277988). To generate this image, wild-type and CASP1 knockout THP-1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 ^°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-Caspase-1 antibody [EPR19672] (<a href='/en-us/products/primary-antibodies/caspase-1-antibody-epr19672-ab207802'>ab207802</a>) at 1/1000 dilution

Lane 1:

Wild-type THP-1 cell lysate at 40 µg

Lane 2:

CASP1 knockout THP-1 cell lysate at 40 µg

Lane 2:

Western blot - Human CASP1 (Caspase-1) knockout THP-1 cell line (<a href='/en-us/products/cell-lines/human-casp1-caspase-1-knockout-thp-1-cell-line-ab276116'>ab276116</a>)

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

HEK-293 cell lysate at 20 µg

Predicted band size: 45 kDa

Observed band size: 35-40 kDa,48 kDa

false

• WB

Lab

Western blot - Human CASP1 (Caspase-1) knockout THP-1 cell lysate (AB277988)

Lane 1 : Wild-type THP-1 cell lysate 40 μg

Lane 2 : THP-1 cell lysate 40 μg

Lane 3 : HeLa cell lysate 20 μg

Lane 4 : HEK-293 cell lysate 20 μg

False colour image of Western blot : Anti-CASP1 antibody staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, the antibody was shown to bind specifically to CASP1. A band was observed at 35-40, 48 kDa in wild-type THP-1 cell lysates with no signal observed at this size in CASP1 knockout cell line ab276116 (knockout cell lysate ab277988). To generate this image, wild-type and CASP1 knockout THP-1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Anti-CASP1 antibody at 1/1000 dilution

Lane 1:

Wild-type THP-1 cell lysate at 40 µg

Lane 2:

THP-1 cell lysate at 40 µg

Lane 2:

Western blot - Human CASP1 (Caspase-1) knockout THP-1 cell line (<a href='/en-us/products/cell-lines/human-casp1-caspase-1-knockout-thp-1-cell-line-ab276116'>ab276116</a>)

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

HEK-293 cell lysate at 20 µg

false