Human CASP7 (Caspase-7) knockout HeLa cell lysate
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CASP7 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 13 bp deletion in exon2 and 1 bp deletion in exon2 and 20 bp deletion in exon2.
View Alternative Names
Apoptotic protease Mch-3, CASP7_HUMAN, CMH-1, Caspase 7, Caspase 7 apoptosis related cysteine peptidase, Caspase-7 subunit p11, ICE-LAP3, ICE-like apoptotic protease 3, LICE2, MCH3
- WB
Unknown
Western blot - Human CASP7 (Caspase-7) knockout HeLa cell lysate (AB257380)
Lane 1 : Wild-type HeLa cell lysate (20µg)
Lane 2 : CASP7 knockout HeLa cell lysate (20µg)
Lanes 1- 2 : Merged signal (red and green). Green - ab32522 observed at 38 kDa. Red - loading control ab7291 observed at 50 kDa.
ab32522 Recombinant Anti-HMGB1 antibody [EPR3506] was shown to specifically react with pro Caspase-7 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265777 (knockout cell lysate ab257380) was used. Wild-type and pro Caspase-7 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab32522 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Caspase-7 antibody [E22] (<a href='/en-us/products/primary-antibodies/caspase-7-antibody-e22-ab32522'>ab32522</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
CASP7 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human CASP7 (Caspase-7) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-casp7-caspase-7-knockout-hela-cell-line-ab265777'>ab265777</a>)
Predicted band size: 34 kDa
Observed band size: 38 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human CASP7 (Caspase-7) knockout HeLa cell lysate (AB257380)
Allele-1 : 20 bp deletion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human CASP7 (Caspase-7) knockout HeLa cell lysate (AB257380)
Allele-3 : 1 bp deletion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human CASP7 (Caspase-7) knockout HeLa cell lysate (AB257380)
Allele-2 : 13 bp deletion in exon2
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB255818
Anti-Caspase-7 antibody [EPR22839-12]
BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated
![Western blot - Anti-Caspase-7 antibody [EPR22839-12] (AB255818)](https://content.abcam.com/products/images/caspase-7-antibody-epr22839-12-ab255818--western-blot-img10043.jpg)
- 0
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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