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AB263806

Human CAV1 (Caveolin-1) knockout HeLa cell lysate

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CAV1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and Insertion of the selection cassette in exon 1.

View Alternative Names

BSCL3, CAV, CAV1_HUMAN, CGL3, Caveolin 1 caveolae protein 22kDa, Caveolin-1, LCCNS, MSTP085, OTTHUMP00000025031, PPH3, VIP 21, caveolae protein, 22 kD, caveolin 1 alpha isoform, caveolin 1 beta isoform, cell growth-inhibiting protein 32

5 Images
Western blot - Human CAV1 (Caveolin-1) knockout HeLa cell lysate (AB263806)
  • WB

Lab

Western blot - Human CAV1 (Caveolin-1) knockout HeLa cell lysate (AB263806)

Lane 1 : A431 cell lysate (20 μg)

Lane 2 : A549 cell lysate (20 μg)

Lane 3 : Wild-type HeLa cell lysate (20 μg)

Lane 4 : CAV1 knockout HeLa cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab192869 observed at 20 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab192869 was shown to react with Caveolin-1 in wild-type HeLa. Loss of signal was observed when knockout cell line ab255371 (knockout cell lysate ab263806) was used. Wild-type and Caveolin-1 knockout samples were subjected to SDS-PAGE. ab192869 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 10000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Caveolin-1 antibody [EPR15554] - BSA and Azide free (<a href='/en-us/products/primary-antibodies/caveolin-1-antibody-epr15554-bsa-and-azide-free-ab240332'>ab240332</a>) at 1/10000 dilution

Lane 1:

A431 cell lysate at 20 µg

Lane 2:

A549 cell lysate at 20 µg

Lane 3:

Wild-type HeLa cell lysate at 20 µg

Lane 4:

Caveolin-1 knockout HeLa cell lysate at 20 µg

Predicted band size: 20 kDa

Observed band size: 20 kDa

false

Western blot - Human CAV1 (Caveolin-1) knockout HeLa cell lysate (AB263806)
  • WB

Lab

Western blot - Human CAV1 (Caveolin-1) knockout HeLa cell lysate (AB263806)

Lane 1 : Wild-type HeLa cell lysate 20 ug
Lane 2 : CAV1 knockout HeLa cell lysate 20 ug
Lane 3 : A431 cell lysate 20 ug
Lane 4 : A549 cell lysate 20 ug
Lanes 1 - 4 : Merged signal (red and green). Green - ab193893 observed at 20 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab193893 was shown to react with Caveolin-1 in wild-type HeLa cells in Western blot with loss of signal observed in CAV1 knockout cell line ab255371 (CAV1 knockout cell lysate ab263806). Wild-type HeLa and CAV1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab193893 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 5000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - HRP Anti-Caveolin-1 antibody [E249] - Caveolae Marker (<a href='/en-us/products/primary-antibodies/hrp-caveolin-1-antibody-e249-caveolae-marker-ab193893'>ab193893</a>) at 1/5000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

CAV1 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human CAV1 (Caveolin-1) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-cav1-caveolin-1-knockout-hela-cell-line-ab255371'>ab255371</a>)

Lane 3:

A431 cell lysate at 20 µg

Lane 4:

A549 cell lysate at 20 µg

Predicted band size: 20 kDa

Observed band size: 20 kDa

false

Exposure time: 90s

Western blot - Human CAV1 (Caveolin-1) knockout HeLa cell lysate (AB263806)
  • WB

Lab

Western blot - Human CAV1 (Caveolin-1) knockout HeLa cell lysate (AB263806)

Lane 1 : A431 cell lysate (20 μg)

Lane 2 : A549 cell lysate (20 μg)

Lane 3 : Wild-type HeLa cell lysate (20 μg)

Lane 4 : CAV1 knockout HeLa cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab32577 observed at 20 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab32577 was shown to react with Caveolin-1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab255371 (knockout cell lysate ab263806) was used. Wild-type and Caveolin-1 knockout samples were subjected to SDS-PAGE. ab32577 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (<a href='/en-us/products/primary-antibodies/caveolin-1-antibody-e249-caveolae-marker-ab32577'>ab32577</a>) at 1/1000 dilution

Lane 1:

A431 cell lysate at 20 µg

Lane 2:

A549 cell lysate at 20 µg

Lane 2:

Western blot - Human CAV1 (Caveolin-1) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-cav1-caveolin-1-knockout-hela-cell-line-ab255371'>ab255371</a>)

Lane 3:

Wild-type HeLa cell lysate at 20 µg

Lane 4:

CAV1 knockout HeLa cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Predicted band size: 20 kDa

Observed band size: 20 kDa,37 kDa

false

Sanger Sequencing - Human CAV1 (Caveolin-1) knockout HeLa cell lysate (AB263806)
  • Sanger seq

Unknown

Sanger Sequencing - Human CAV1 (Caveolin-1) knockout HeLa cell lysate (AB263806)

Allele-2 : Insertion of the selection cassette in exon 1

Sanger Sequencing - Human CAV1 (Caveolin-1) knockout HeLa cell lysate (AB263806)
  • Sanger seq

Unknown

Sanger Sequencing - Human CAV1 (Caveolin-1) knockout HeLa cell lysate (AB263806)

Allele-1 : 1 bp insertion in exon 1

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and Insertion of the selection cassette in exon 1.

Disease

Adenocarcinoma

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab263806-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human CAV1 knockout HeLa cell lysate", "number":"AB263806-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HeLa cell lysate", "number":"AB263806-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Gene name
CAV1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Caveolin-1 also known as Cav-1 is an integral membrane protein with a molecular weight of approximately 22 kDa. It functions mechanically as a scaffolding protein in caveolae which are tiny invaginations in the plasma membrane of many cell types. These structures are particularly abundant in adipocytes endothelial cells and muscle cells. Caveolin-1 anchors itself to the cell membrane and plays a role in organizing and concentrating certain signaling molecules.
Biological function summary

Caveolae provide a platform for various signaling pathways and involve caveolin-1 as a major component. Caveolin-1 interacts with multiple signaling molecules such as G-protein coupled receptors and Src family kinases to modulate signal transduction. This protein forms part of a larger caveolar complex contributing to cellular processes including endocytosis and lipid regulation. Its presence as a marker in caveolae highlights its significance in cellular functions.

Pathways

Caveolin-1 influences the insulin signaling and nitric oxide (NO) signaling pathways. In the insulin signaling pathway caveolin-1 interacts with insulin receptors to modulate glucose uptake. It also associates with eNOS (endothelial nitric oxide synthase) in the NO signaling pathway impacting vascular function. These relationships highlight its role in cellular communication and regulatory mechanisms within the human body.

Caveolin-1 is involved in cancer and cardiovascular diseases. In cancer altered expression of caveolin-1 contributes to tumor progression while in cardiovascular diseases it affects endothelial function through its interaction with eNOS. The connection of caveolin-1 to proteins like insulin receptors and eNOS reveals its critical influence in these conditions providing potential targets for therapeutic intervention.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Complementary Products

Cell Lines & Lysates

AB255371

Human CAV1 (Caveolin-1) knockout HeLa cell line

Advanced Validation

Immunocytochemistry - Human CAV1 (Caveolin-1) knockout HeLa cell line (AB255371)

  • 0
  • 0
Primary Antibodies

AB32577

Anti-Caveolin-1 antibody [E249] - Caveolae Marker

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (AB32577)

  • 5
  • 2
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Proteins & Peptides

AB217407

Recombinant human Vitronectin/S-Protein (Animal Free)

  • 0
  • 0

Product promise

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