Human CBLL1 knockout HeLa cell lysate
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CBLL1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon6.
View Alternative Names
CasBrM (murine) ecotropic retroviral transforming sequencelike 1, Casitas B-lineage lymphoma-like 1, Casitas B-lineage lymphoma-transforming sequence-like protein 1, E3 ubiquitin-protein ligase Hakai, Ecadherin binding protein E7, FLJ23109, HAKAI_HUMAN, MGC163401, MGC163403, OTTHUMP00000206720, OTTHUMP00000206722, OTTHUMP00000206724, RING finger protein 188, RNF188, c-Cbl-like protein 1, cbll1
- Sanger seq
Unknown
Sanger Sequencing - Human CBLL1 knockout HeLa cell lysate (AB258805)
Homozygous : 1 bp insertion in exon6
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CBLL1 plays a significant role in cellular processes such as endocytosis cell adhesion and regulation of epithelial-mesenchymal transition (EMT). CBLL1 functions as part of a complex that helps target proteins for degradation through the ubiquitin-proteasome system. This activity is especially relevant in the regulation of cell signaling pathways where it targets various substrates such as E-cadherin for ubiquitination.
Pathways
CBLL1 is important in modulating signaling cascades like the Wnt and TGF-beta pathways. By influencing these pathways CBLL1 interacts with proteins like beta-catenin and SMADs. The degradation of E-cadherin by CBLL1 facilitates cellular detachment and migration a critical step in cellular processes regulated by these pathways.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB316307
Anti-CBLL1 antibody [EPR25389-121] - BSA and Azide free
BOND RX™ Validated|Recombinant|RabMAb
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBLL1 antibody [EPR25389-121] - BSA and Azide free (AB316307)](https://content.abcam.com/products/images/cbll1-antibody-epr25389-121-bsa-and-azide-free-ab316307--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427465.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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