Human CBX1 (HP1 beta) knockout HEK-293T cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
CBX1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon2.
View Alternative Names
CBX, CBX1_HUMAN, Chromobox 1, Chromobox homolog 1 (HP1 beta homolog Drosophila ), Chromobox protein homolog 1, Drosophila HP1 beta, HP1 beta, HP1 beta homolog, HP1 beta homolog Drosophila, HP1, Drosophila. homolog of, beta, HP1Hs beta, Heterochromatin protein 1 beta, Heterochromatin protein 1 homolog beta, Heterochromatin protein p25, Human heterochromatin protein p25 mRNA complete cds, M31, MOD 1, Modifier 1 protein, chromobox homolog 1, heterochromatin protein p25 beta, p25beta
- WB
Lab
Western blot - Human CBX1 (HP1 beta) knockout HEK-293T cell lysate (AB257383)
Lane 1 : Wild-type HEK-293T cell lysate (20µg)
Lane 2 : CBX1 knockout HEK-293T cell lysate (20µg)
Lanes 1- 2 : Merged signal (red and green). Green - ab10478 observed at 26 kDa. Red - loading control ab7291 observed at 50 kDa.
ab10478 Anti-CBX1 / HP1 beta antibody was shown to specifically react with CBX1 / HP1 beta in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266356 (knockout cell lysate ab257383) was used. Wild-type and CBX1 / HP1 beta knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab10478 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-CBX1 / HP1 beta antibody (<a href='/en-us/products/primary-antibodies/cbx1-hp1-beta-antibody-ab10478'>ab10478</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK-293T cell lysate at 20 µg
Lane 2:
CBX1 knockout HEK-293T cell lysate at 20 µg
Lane 2:
Western blot - Human CBX1 (HP1 beta) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-cbx1-hp1-beta-knockout-hek-293t-cell-line-ab266356'>ab266356</a>)
Predicted band size: 21 kDa
Observed band size: 26 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human CBX1 (HP1 beta) knockout HEK-293T cell lysate (AB257383)
Homozygous : 10 bp deletion in exon2
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The CBX1 protein acts as an integral component of the chromatin remodeling complex. By binding to methylated histones CBX1 helps stabilize chromatin architecture and repress transcription of genes within heterochromatic regions. This function is essential for maintaining gene silencing which is critical for processes like development and differentiation. CBX1 collaborates with other HP1 isoforms such as HP1 alpha and HP1 gamma as part of the family of heterochromatin proteins that contribute collectively to chromatin dynamics and gene expression control.
Pathways
CBX1 plays a part in the epigenetic transcriptional repression and cell cycle regulation pathways. It associates with SUV39H1 a methyltransferase to recognize and bind the H3K9me3 mark adding stability to the methylation signal and contributing to gene silencing. Additionally CBX1 interacts with other proteins like DNMT1 a DNA methyltransferase reinforcing its role in sustaining repressive chromatin states and preserving genome integrity during DNA replication.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com