JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB257110

Human CBX3 (HP1 gamma) knockout HeLa cell lysate

Be the first to review this product! Submit a review

|

(0 Publication)

CBX3 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 4 bp deletion in exon 2 and 5 bp deletion in exon 2.

View Alternative Names

HECH, Heterochromatin protein 1 homolog gamma, Chromobox protein homolog 3, HP1 gamma, Modifier 2 protein, CBX3

3 Images
Western blot - Human CBX3 (HP1 gamma) knockout HeLa cell lysate (AB257110)
  • WB

Lab

Western blot - Human CBX3 (HP1 gamma) knockout HeLa cell lysate (AB257110)

Lane 1 : Wild-type HeLa cell lysate (20µg)

Lane 2 : CBX3 knockout HeLa cell lysate (20µg)

Lanes 1- 2 : Merged signal (red and green). Green - ab217999 observed at 25 kDa. Red - loading control ab7291 observed at 50 kDa.

ab217999 Anti-HP1 gamma/CBX3 antibody [EPR19802] was shown to specifically react with HP1 gamma/CBX3 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261744 (knockout cell lysate ab257110) was used. Wild-type and HP1 gamma/CBX3 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab217999 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 2000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-HP1 gamma/CBX3 antibody [EPR19802] (<a href='/en-us/products/primary-antibodies/hp1-gamma-cbx3-antibody-epr19802-ab217999'>ab217999</a>) at 1/2000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

CBX3 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human CBX3 (HP1 gamma) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-cbx3-hp1-gamma-knockout-hela-cell-line-ab261744'>ab261744</a>)

Predicted band size: 20 kDa

Observed band size: 25 kDa

false

Sanger Sequencing - Human CBX3 (HP1 gamma) knockout HeLa cell lysate (AB257110)
  • Sanger seq

Unknown

Sanger Sequencing - Human CBX3 (HP1 gamma) knockout HeLa cell lysate (AB257110)

Allele-2 : 4 bp deletion in exon 2

Sanger Sequencing - Human CBX3 (HP1 gamma) knockout HeLa cell lysate (AB257110)
  • Sanger seq

Unknown

Sanger Sequencing - Human CBX3 (HP1 gamma) knockout HeLa cell lysate (AB257110)

Allele-1 : 5 bp deletion in exon 2

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 4 bp deletion in exon 2 and 5 bp deletion in exon 2.

Disease

Adenocarcinoma

style
left-column

Complementary Products

Primary Antibodies

AB217999

Anti-HP1 gamma/CBX3 antibody [EPR19802]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HP1 gamma/CBX3 antibody [EPR19802] (AB217999)

  • 0
  • 0
Proteins & Peptides

AB124548

Recombinant Human HP1 gamma/CBX3 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human HP1 gamma/CBX3 protein (His tag N-Terminus) (AB124548)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

style
left-column

What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab257110-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human CBX3 knockout HeLa cell lysate", "number":"AB257110-CMP02", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HeLa cell lysate", "number":"AB257110-CMP01", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Gene name
CBX3
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The HP1 gamma protein also known as CBX3 is an essential component of the chromatin organization machinery. It has a molecular weight of approximately 22-25 kDa. HP1 gamma is expressed in a variety of tissues including brain liver and muscle indicating its broad functional involvement across different biological systems. It exhibits a high affinity for binding to histone H3 when it is methylated facilitating the formation of heterochromatin which is key to regulating gene expression and maintaining genome stability.
Biological function summary

HP1 gamma plays a significant role in gene silencing and chromatin structure maintenance. It forms part of a protein complex that includes chromatin remodeling enzymes and transcriptional repressors which help regulate access to genomic DNA. HP1 gamma's association with other chromatin-associated proteins helps modulate the transcriptional response to cellular stimuli ensuring appropriate gene expression patterns needed for normal cell function and development.

Pathways

HP1 gamma is involved in heterochromatin formation and maintenance pathways working alongside other proteins like SUV39H1 and HP1 alpha. Through these pathways HP1 gamma ensures the compact organization of repetitive DNA sequences and suppresses unnecessary transcription. It is also involved in the DNA damage response pathway playing a role in preserving genomic integrity by interacting with proteins such as BRCA1 and 53BP1 involved in DNA repair.

HP1 gamma's dysregulation has been associated with cancer and neurological disorders. Overexpression or mutations in this protein can lead to aberrant gene silencing contributing to oncogenesis where cancer-related genes become improperly regulated. Additionally alterations in HP1 gamma function are linked to neurodegenerative diseases potentially through its interaction with proteins like MECP2 whereby changes in the chromatin environment influence neuronal gene expression and function. Therefore understanding HP1 gamma's role provides insights into potential therapeutic targets for these conditions.
style
left-column

Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

style
left-column

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

style
left-column

Product protocols

style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com