Human CCDC137 (CC137) knockout HeLa cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human CCDC137 (CC137) knockout HeLa cell lysate (AB263137)
Allele-2 : 1 bp insertion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human CCDC137 (CC137) knockout HeLa cell lysate (AB263137)
Allele-1 : 1 bp deletion in exon1
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CC137 is essential for modulating immune responses and is part of the chemokine family which regulates the migration of leukocytes. CC137 can form part of a larger cytokine signaling complex enhancing the communication between immune cells. It is involved in the regulation of inflammatory responses by activating specific receptors on the surface of target cells which leads to the activation of intracellular signaling pathways.
Pathways
CC137 participates in the chemokine signaling pathway and the JAK-STAT signaling pathway. It plays a role in the recruitment and activation of immune cells through interactions with chemokine receptors like CCR2 and CCR3. These pathways are important for movements and activation of various immune cell types linking CC137 to proteins such as JAK2 and STAT3 which are integral to signal transduction processes.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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