Human CCL4 knockout THP-1 cell lysate
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CCL4L1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 7 bp deletion in exon 1.
View Alternative Names
ACT-2, AT744.1, AT744.2, C C motif chemokine 4, C C motif chemokine 4 like, C C motif chemokine ligand 4 like 1, C C motif chemokine ligand 4 like 2, CC chemokine ligand 4, CC chemokine ligand 4L1, CC chemokine ligand 4L1d2, CC chemokine ligand 4L2, CCL4L, CCL4_HUMAN, Chemokine (C C motif) ligand 4, Chemokine (C C motif) ligand 4 like 1, Chemokine (C C motif) ligand 4 like 1, telomeric, Chemokine (C C motif) ligand 4 like 2, Chemokine CC Motif Ligand 4, G 26, G-26 T-lymphocyte-secreted protein, HC21, Immune activation 2, LAG-1, Lymphocyte activation gene 1, Lymphocyte activation gene 1 protein, MGC104418, MGC126025, MGC126026, MIP-1-beta, MIP-1-beta(1-69), MIP-1-beta(3-69), MIP1B, MIP1B1, Macrophage inflammatory protein 1-beta, Macrophage inflammatory protein 1b2, Monocyte adherence induced protein 5 alpha, PAT 744, Protein H400, SCYA2, SCYA4, SCYA4L, SCYA4L1, SCYA4L2, SCYQ4L2, SIS-gamma, Secreted protein G 26, Small inducible cytokine A4 (homologous to mouse Mip 1b), Small-inducible cytokine A4, T-cell activation protein 2, ccl4l 1, small inducible cytokine A4-like
- WB
Lab
Western blot - Human CCL4 knockout THP-1 cell lysate (AB275512)
Lane 1 : Wild-type THP-1 Vehicle control + Brefeldin A (5 μ/mL, 6 h) cell lysate 20 μg
Lane 2 : Wild-type THP-1 Treated PMA (100 ng/mL, 56 h) + LPS (1 μ/mL, 24 h) + Brefeldin A (5 μ/mL, 6 h) cell lysate 20 μg
Lane 3 : CCL4 knockout THP-1 Vehicle control + Brefeldin A (5 μ/mL, 6 h) cell lysate 20 μg
Lane 4 : CCL4 knockout THP-1 Treated PMA (100 ng/mL, 56 h) + LPS (1 μ/mL, 24 h) + Brefeldin A (5 μ/mL, 6 h) cell lysate 20 μg
False colour image of Western blot : Anti-CCL4/MIP-1 beta antibody [EP521Y] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab45690 was shown to bind specifically to CCL4/MIP-1 beta. A band was observed at 12 kDa in wild-type THP-1 cell lysates with no signal observed at this size in CCL4 knockout cell line ab273719 (knockout cell lysate ab275512). To generate this image, wild-type and CCL4 knockout THP-1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-CCL4/MIP-1 beta antibody [EP521Y] (<a href='/en-us/products/primary-antibodies/ccl4-mip-1-beta-antibody-ep521y-ab45690'>ab45690</a>) at 1/1000 dilution
Lane 1:
Wild-type THP-1 Vehicle control + Brefeldin A (5 u/mL, 6 h) cell lysate at 20 µg
Lane 2:
Wild-type THP-1 Treated PMA (100 ng/mL, 56 h) + LPS (1 u/mL, 24 h) + Brefeldin A (5 u/mL, 6 h) cell lysate at 20 µg
Lane 2:
Western blot - Human CCL4 knockout THP-1 cell line (<a href='/en-us/products/cell-lines/human-ccl4-knockout-thp-1-cell-line-ab273719'>ab273719</a>)
Lane 3:
CCL4 knockout THP-1 Vehicle control + Brefeldin A (5 u/mL, 6 h) cell lysate at 20 µg
Lane 4:
CCL4 knockout THP-1 Treated PMA (100 ng/mL, 56 h) + LPS (1 u/mL, 24 h) + Brefeldin A (5 u/mL, 6 h) cell lysate at 20 µg
Predicted band size: 10 kDa
Observed band size: 12 kDa
false
- NGS
Lab
Next Generation Sequencing - Human CCL4 knockout THP-1 cell lysate (AB275512)
Allele-1 : 7 bp deletion in exon 1
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
Treatments:
Human CCL4 knockout THP-1 cell lysate - Brefeldin A (5 μg/ml, 6h)
Human wild-type THP-1 cell lysate - Brefeldin A (5 μg/ml, 6h)
Human CCL4 knockout THP-1 cell lysate - PMA (100 ng/ml, 56h), LPS (1 μg/ml, 24h) and Brefeldin A (5 μg/ml, during the last 6h of LPS treatment)
Human wild-type THP-1 cell lysate - PMA (100 ng/ml, 56h), LPS (1 μg/ml, 24h) and Brefeldin A (5 μg/ml, during the last 6h of LPS treatment)
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CCL4 is critical in immune system modulation. It coordinates leukocyte activation and trafficking contributing to inflammation and immune surveillance. It does not function in isolation but rather interacts with other chemokines and receptors. CCL4 alongside its sibling chemokines forms a dynamic network that ensures a balanced immune response. It connects to receptors such as CCR5 playing substantial roles in its activities.
Pathways
CCL4 participates in essential signaling cascades that mediate immune responses. It is notably part of the chemokine signaling pathway which is instrumental in directing cell movement. CCL4 also engages with proteins like CCR5 in the inflammatory and immune response pathways. This interaction enhances communication between cells required for effective pathogen defense.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Suspension
Gender
Male
Complementary Products
Primary Antibodies
AB45690
Anti-CCL4/MIP-1 beta antibody [EP521Y]
RabMAb|Recombinant|KO Validated
![Immunocytochemistry/ Immunofluorescence - Anti-CCL4/MIP-1 beta antibody [EP521Y] (AB45690)](https://content.abcam.com/products/images/ccl4-mip-1-beta-antibody-ep521y-ab45690--immunocytochemistry-immunofluorescence-img90733.jpg)
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Product promise
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