Human CCND1 (Cyclin D1) knockout HeLa cell lysate

• WB

Lab

Western blot - Human CCND1 (Cyclin D1) knockout HeLa cell lysate (AB256864)

Lane 1 : Wild-type HeLa cell lysate (20µg)

Lane 2 : CCND1 knockout HeLa cell lysate (20µg)

Lanes 1- 2 : Merged signal (red and green). Green - ab40754 observed at 36 kDa. Red - loading control ab7291 observed at 50 kDa.

ab40754 Anti-Cyclin D1 antibody [EP272Y] was shown to specifically react with Cyclin D1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261760 (knockout cell lysate ab256864) was used. Wild-type and Cyclin D1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab40754 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4^°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Cyclin D1 antibody [EP272Y] (ab40754) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

CCND1 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human CCND1 (Cyclin D1) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-ccnd1-cyclin-d1-knockout-hela-cell-line-ab261760'>ab261760</a>)

Predicted band size: 34 kDa

Observed band size: 36 kDa

false

• WB

Lab

Western blot - Human CCND1 (Cyclin D1) knockout HeLa cell lysate (AB256864)

Lane 1 : Wild-type HeLa cell lysate (20µg)

Lane 2 : CCND1 knockout HeLa cell lysate (20µg)

Lanes 1- 2 : Merged signal (red and green). Green - ab16663 observed at 36 kDa. Red - loading control ab7291 observed at 50 kDa.

ab16663 Anti-Cyclin D1 antibody [SP4] was shown to specifically react with Cyclin D1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261760 (knockout cell lysate ab256864) was used. Wild-type and Cyclin D1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab16663 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4^°C at 1 in 200 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Cyclin D1 antibody [SP4] (<a href='/en-us/products/primary-antibodies/cyclin-d1-antibody-sp4-ab16663'>ab16663</a>) at 1/200 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

CCND1 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human CCND1 (Cyclin D1) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-ccnd1-cyclin-d1-knockout-hela-cell-line-ab261760'>ab261760</a>)

Predicted band size: 34 kDa

Observed band size: 36 kDa

false

• Sanger seq

Unknown

Sanger Sequencing - Human CCND1 (Cyclin D1) knockout HeLa cell lysate (AB256864)

Homozygous : Insertion of the selection cassette in exon 1