Human CCND2 (Cyclin D2) knockout HEK-293T cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
CCND2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon1 and 8 bp insertion in exon1.
View Alternative Names
CCND2_HUMAN, CyclinD2, G1/S-specific cyclin-D2, KIAK0002, MGC102758, MPPH3
- Sanger seq
Unknown
Sanger Sequencing - Human CCND2 (Cyclin D2) knockout HEK-293T cell lysate (AB257875)
Allele-1 : 2 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human CCND2 (Cyclin D2) knockout HEK-293T cell lysate (AB257875)
Allele-2 : 8 bp insertion in exon1
- WB
Lab
Western blot - Human CCND2 (Cyclin D2) knockout HEK-293T cell lysate (AB257875)
All lanes:
Western blot - Anti-Cyclin D2 antibody [EPR19659] (<a href='/en-us/products/primary-antibodies/cyclin-d2-antibody-epr19659-ab207604'>ab207604</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK-293T cell lysate at 20 µg
Lane 2:
Western blot - Human CCND2 (Cyclin D2) knockout HEK-293T cell lysate (ab257875) at 20 µg
Lane 2:
Western blot - Human CCND2 (Cyclin D2) knockout HEK-293T cell lysate (ab257875)
Lane 2:
Western blot - Human CCND2 (Cyclin D2) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-ccnd2-cyclin-d2-knockout-hek-293t-cell-line-ab267318'>ab267318</a>)
Lane 3:
HeLa cell lysate at 20 µg
Lane 4:
U-2 OS cell lysate at 20 µg
Secondary
Lanes 1 - 4:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution
Lanes 1 - 4:
Western blot - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>) at 1/20000 dilution
Predicted band size: 33 kDa
Observed band size: 33 kDa
false
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
![Western blot - Anti-Cyclin D2 antibody [EPR19659] (AB207604)](https://content.abcam.com/products/images/cyclin-d2-antibody-epr19659-ab207604--western-blot-img25478.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com