Human CCNL2 knockout HEK-293T cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human CCNL2 knockout HEK-293T cell lysate (AB263139)
Homozygous : 1 bp deletion in exon 1
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Cyclin L2 modulates transcription processes by associating with splicing factors and RNA polymerase II. It is a part of the spliceosomal complex which influences mRNA processing by ensuring proper splicing of pre-mRNA. This regulation is important for generating diverse and functionally appropriate mRNA transcripts which influences cellular outcome and function. CCNL2's capability to bind with cdk kinases further enhances its role in managing transcription and cell cycle checkpoints within cells.
Pathways
CCNL2 is involved in significant cellular processes such as the cell cycle and mRNA processing pathways. A notable pathway includes the RNA splicing pathway where Cyclin L2 plays a role alongside proteins like cdk kinases which phosphorylate the C-terminal domain to facilitate splicing. Additionally it contributes to the regulation of the cell cycle coordinating with proteins such as cyclin-dependent kinases (CDK) to ensure proper cell cycle progression and division.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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