Human CCP110 (CP110) knockout HEK-293T cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human CCP110 (CP110) knockout HEK-293T cell lysate (AB258345)
Homozygous : 10 bp deletion in exon 4
Complementary Products
Primary Antibodies
AB179503
Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker
RabMAb|Recombinant|Lab Essentials
![Western blot - Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker (AB179503)](https://content.abcam.com/products/images/gamma-tubulin-antibody-epr16793-centrosome-marker-ab179503--western-blot-img56352.jpg)
- 4
- 3
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CP110 interacts with several other proteins to regulate centrosome function and cell division. It is a part of the centriole duplication complex which is essential for proper ciliogenesis and cell cycle control. CP110 caps the mother centriole and prevents the formation of cilia when the cell is in a dividing state therefore playing a role in the balance between cell division and the formation of cilia which contribute to cellular signaling and sensory functions.
Pathways
The centriole duplication and ciliogenesis processes involve CP110 as it interacts with pathways such as the cell cycle and the Sonic Hedgehog signaling pathway. CP110 partners with proteins like CEP97 and CEP290 to manage the length of centrioles and assist in the timely progression of the cell cycle. The regulation by CP110 ensures that centrosome duplication happens correctly preventing errors that might lead to cell division abnormalities.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com