Human CD164 knockout HEK-293T cell lysate
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CD164 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 148 bp deletion in exon 1.
View Alternative Names
CD164 antigen, CD164 antigen, sialomucin, CD164 molecule, sialomucin, ESM, MGC 24, Multi glycosylated core protein 24, Putative mucin core protein 24 precursor, Putative mucin core protein precursor 24, RP11-425D10.9, Sialomucin CD164, endolyn, sialomucin
- Sanger seq
Unknown
Sanger Sequencing - Human CD164 knockout HEK-293T cell lysate (AB263140)
Homozygous : 148 bp deletion in exon 1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CD164 influences the adhesive properties within specific cellular environments. It contributes to the formation of complexes with other cellular proteins to modulate cell communication and migration. The interaction of CD164 with integrins and cell surface receptors regulates cellular trafficking. It influences the behavior of cells at high confluency which impacts how these cells aggregate and differentiate in tissue structures.
Pathways
More than four biochemical interactions influence CD164's role in controlling cellular movement and tissue architecture. It plays a significant role in the chemokine signaling pathway affecting memory B-cell homing. Furthermore CD164 is linked with the transforming growth factor-beta (TGF-beta) pathway through its association with integrins which regulates numerous cellular processes such as growth and development. By modulating these pathways it participates in the body's response to injury and inflammation.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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