Human CD167a/DDR1 knockout HeLa cell lysate
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DDR1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 47 bp insertion in exon12 and Insertion of the selection cassette in exon12.
View Alternative Names
CAK, CD 167, CD167 antigen-like family member A, CD167a, Cell adhesion kinase, DDR, DDR1_HUMAN, Discoidin domain receptor, Discoidin domain receptor tyrosine kinase 1, Discoidin receptor tyrosine kinase, Discoidin receptor tyrosine kinase isoform a, EDDR 1, Epithelial discoidin domain receptor 1, Epithelial discoidin domain-containing receptor 1, Epithelial specific receptor kinase, HGK2, MCK-10, Mammarian carcinoma kinase 10, Mammary carcinoma kinase 10, NTRK 4, Neuroepithelial tyrosine kinase, Neurotrophic tyrosine kinase receptor type 4, OTTHUMP00000029343, OTTHUMP00000029344, OTTHUMP00000029345, OTTHUMP00000029346, OTTHUMP00000029347, OTTHUMP00000164863, OTTHUMP00000164867, OTTHUMP00000222080, PTK 3, PTK 3A protein tyrosine kinase 3A, PTK3A, Protein-tyrosine kinase 3A, Protein-tyrosine kinase RTK-6, RTK 6, Receptor tyrosine kinase NEP, TRK E, Tyrosine kinase DDR, Tyrosine kinase receptor E, Tyrosine-protein kinase CAK
- Sanger seq
Unknown
Sanger Sequencing - Human CD167a/DDR1 knockout HeLa cell lysate (AB258840)
Allele-2 : Insertion of the selection cassette in exon12
- Sanger seq
Unknown
Sanger Sequencing - Human CD167a/DDR1 knockout HeLa cell lysate (AB258840)
Allele-1 : 47 bp insertion in exon12
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CD167a/DDR1 modulates interactions between cells and their surrounding matrix. It is involved in a complex with collagen mediating signal transduction processes. This interaction is important for cellular biophysical responses driving processes like cell migration and differentiation. In marmoset thyroid tissue DDR1 helps maintain structural integrity and regulates cellular activities important for normal thyroid function.
Pathways
CD167a/DDR1 participates in key signaling pathways including those related to cell adhesion and extracellular matrix remodeling. Specifically it operates within the MAPK/ERK pathway influencing cell cycle dynamics and responses to stress signals. DDR1 also interacts with proteins like SHC and Ras enabling communication between the cell surface and intracellular targets necessary for growth and adaptation.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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