Human CD791 knockout Raji cell lysate

• WB

Lab

Western blot - Human CD791 knockout Raji cell lysate (AB281361)

Lane 1 : Wild-type Raji cell lysate 20 μg
Lane 2 : CD79A knockout Raji cell lysate 20 μg
Lane 3 : Daudi cell lysate 20 μg
Lane 4 : HAP1 cell lysate 20 μg
False colour image of Western blot : Anti-CD79a antibody [EP3618] staining at 1/5000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab79414 was shown to bind specifically to CD79a. A band was observed at 40-50 kDa in wild-type Raji cell lysates with no signal observed at this size in CD79A knockout cell line ab274911 (knockout cell lysate ab281361). To generate this image, wild-type and CD79A knockout Raji cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L ^{(IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.}

All lanes:

Western blot - Anti-CD79a antibody [EP3618] (<a href='/en-us/products/primary-antibodies/cd79a-antibody-ep3618-ab79414'>ab79414</a>) at 1/5000 dilution

Lane 1:

Wild-type Raji cell lysate at 20 µg

Lane 2:

CD79A knockout Raji cell lysate at 20 µg

Lane 2:

Western blot - Human CD791 knockout Raji cell line (<a href='/en-us/products/cell-lines/human-cd791-knockout-raji-cell-line-ab274911'>ab274911</a>)

Lane 3:

Daudi cell lysate at 20 µg

Lane 4:

HAP1 cell lysate at 20 µg

Predicted band size: 25 kDa

Observed band size: 40-50 kDa

false

• WB

Lab

Western blot - Human CD791 knockout Raji cell lysate (AB281361)

All lanes:

Western blot - Anti-CD79a antibody [EPR3619] (<a href='/en-us/products/primary-antibodies/cd79a-antibody-epr3619-ab133483'>ab133483</a>) at 1/1000 dilution

Lane 1:

Wild-type Raji cell lysate at 20 µg

Lane 2:

CD79A knockout Raji cell lysate at 20 µg

Lane 3:

Daudi cell lysate at 20 µg

Secondary

Lanes 1 - 3:

Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution

Lanes 1 - 3:

Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 50 kDa

false