Human CD9 knockout A549 cell lysate
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(0 Publication)
- WB
Lab
Western blot - Human CD9 knockout A549 cell lysate (AB261687)
Lane 1 : Wild-type A549 (Human lung carcinoma cell line) whole cell lysate
Lane 2 : CD9 knockout A549 (Human lung carcinoma cell line) whole cell lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lanes 1 - 3 : Merged signal (red and green). Green - ab92726 observed at 22 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab92726 was shown to recognize CD9 in wild-type A549 cells as signal was lost at the expected MW in CD9 knockout cell line ab261878 (knockout cell lysate ab261687). Additional cross-reactive bands were observed in the wild-type and knockout samples. Wild-type and CD9 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% milk. ab92726 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Anti-CD9 antibody [EPR2949] (<a href='/en-us/products/unavailable/cd9-antibody-epr2949-ab92726'>ab92726</a>) at 1/1000 dilution
Lane 1:
A549 (Human lung carcinoma cell line) whole cell lysate
Lane 2:
CD9 knockout A549 (Human lung carcinoma cell line) whole cell lysate
Lane 3:
MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
false
- NGS
Supplier Data
Next Generation Sequencing - Human CD9 knockout A549 cell lysate (AB261687)
Knockout achieved by CRISPR/Cas9; X = 7 bp deletion, 1 bp deletion; Frameshift = 100%
Complementary Products
Primary Antibodies
AB236630
Anti-CD9 antibody [EPR23105-121]
BOND RX™ Validated|RabMAb|Recombinant|KO Validated
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR23105-121] (AB236630)](https://content.abcam.com/products/images/cd9-antibody-epr23105-121-ab236630--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img62941.jpg)
- 5
- 4
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The CD9 protein is integral to cell adhesion migration and fusion. It forms complexes with other tetraspanins and proteins like EWI-2 and EWI-F contributing to cellular signaling and membrane compartmentalization. Additionally CD9 plays an important role in the formation and secretion of exosomes tiny vesicles emitted by cells that mediate cell-to-cell communication. These exosomes can be analyzed using tools like exosome assays and detection kits that target CD9 proteins.
Pathways
CD9 is involved in the regulation of immune response and cell morphology. It participates in pathways such as the integrin signaling pathway and the phosphatidylinositol 3-kinase (PI3K) signaling pathway. CD9 modulates these interactions by associating with proteins such as integrins and other members of the tetraspanin family influencing cellular movement and proliferation.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com