Human CDC25C knockout HeLa cell lysate
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CDC25C KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2 and 2 bp insertion in exon2.
View Alternative Names
Cdc 25C, Cell division cycle 25 homolog C, Cell division cycle 25C, Cell division cycle 25C protein, Dual specificity phosphatase Cdc25C, M-phase inducer phosphatase 3, MPIP3_HUMAN, Mitosis inducer CDC25, PPP1R60, Phosphotyrosine phosphatase, protein phosphatase 1, regulatory subunit 60
- WB
Unknown
Western blot - Human CDC25C knockout HeLa cell lysate (AB257387)
Lane 1 : Wild-type HeLa cell lysate (20µg)
Lane 2 : CDC25C knockout HeLa cell lysate (20µg)
Lanes 1- 2 : Merged signal (red and green). Green - ab32050 observed at 58 kDa. Red - loading control ab8245 observed at 37 kDa.
ab32050 Anti-Cdc25C antibody [E303] was shown to specifically react with Cdc25C in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265189 (knockout cell lysate ab257387) was used. Wild-type and Cdc25C knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab32050 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Cdc25C antibody [E303] (<a href='/en-us/products/primary-antibodies/cdc25c-antibody-e303-ab32050'>ab32050</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
CDC25C knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human CDC25C knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-cdc25c-knockout-hela-cell-line-ab265189'>ab265189</a>)
Predicted band size: 53 kDa
Observed band size: 58 kDa
false
- WB
Lab
Western blot - Human CDC25C knockout HeLa cell lysate (AB257387)
Lane 1 : Wild-type HeLa cell lysate (20µg)
Lane 2 : CDC25C knockout HeLa cell lysate (20µg)
Lanes 1- 2 : Merged signal (red and green). Green - ab32444 observed at 58 kDa. Red - loading control ab8245 observed at 37 kDa.
ab32444 Anti-Cdc25C antibody [E302] was shown to specifically react with Cdc25C in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265189 (knockout cell lysate ab257387) was used. Wild-type and Cdc25C knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab32444 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Cdc25C antibody [E302] (<a href='/en-us/products/primary-antibodies/cdc25c-antibody-e302-ab32444'>ab32444</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
CDC25C knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human CDC25C knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-cdc25c-knockout-hela-cell-line-ab265189'>ab265189</a>)
Predicted band size: 53 kDa
Observed band size: 58 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human CDC25C knockout HeLa cell lysate (AB257387)
Allele-1 : 1 bp insertion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human CDC25C knockout HeLa cell lysate (AB257387)
Allele-2 : 2 bp insertion in exon2
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The proper function of Cdc25C involves facilitating proper cell cycle progression by coordinating with other cell cycle regulators. It participates in a complex network where it dephosphorylates and activates CDK1/cyclin B1 complexes promoting the mitotic entry. This function is important for maintaining genomic stability during cell division. Misregulation of Cdc25C can lead to cell cycle arrest or uncontrolled cell proliferation highlighting its essential role in cell cycle control mechanisms.
Pathways
Cdc25C fits into the cell cycle checkpoint pathways and is also a part of the DNA damage response pathways. It connects with the p53 and ATM/ATR signaling proteins while responding to DNA damage ensuring a temporary pause in cell cycle progression for repair mechanisms to act. Proper interaction with these pathways is essential for maintaining cellular integrity and preventing the proliferation of damaged cells with CDK1 and Wee1 kinase serving as major interacting proteins in these processes.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc25C antibody [E302] (AB32444)](https://content.abcam.com/products/images/cdc25c-antibody-e302-ab32444--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img71706.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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