Human CDC42 knockout HEK-293T cell lysate
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(0 Publication)
- WB
Lab
Western blot - Human CDC42 knockout HEK-293T cell lysate (AB256868)
Lane 1 : Wild-type HEK-293T cell lysate (20 μg)
Lane 2 : CDC42 knockout HEK-293T cell lysate (20 μg)
Lane 3 : MCF7 cell lysate (20 μg)
Lane 4 : HepG2 cell lysate (20 μg)
Lanes 1-4 : Merged signal (red and green). Green - ab187643 observed at 20 kDa. Red - loading control ab8245 observed at 37 kDa.
ab187643 Anti-CDC42 antibody [EPR15620] was shown to specifically react with CDC42 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266522 (knockout cell lysate ab256868) was used. Wild-type and CDC42 knockout samples were subjected to SDS-PAGE. ab187643 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 5000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-CDC42 antibody [EPR15620] (<a href='/en-us/products/primary-antibodies/cdc42-antibody-epr15620-ab187643'>ab187643</a>) at 1/5000 dilution
Lane 1:
Wild-type HEK-293T cell lysate at 20 µg
Lane 2:
CDC42 knockout HEK-293T cell lysate at 20 µg
Lane 2:
Western blot - Human CDC42 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-cdc42-knockout-hek-293t-cell-line-ab266522'>ab266522</a>)
Lane 3:
MCF7 cell lysate at 20 µg
Lane 4:
HepG2 cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 20 kDa
false
- WB
Unknown
Western blot - Human CDC42 knockout HEK-293T cell lysate (AB256868)
Lane 1 : Wild-type HEK-293T cell lysate (20 μg)
Lane 2 : CDC42 knockout HEK-293T cell lysate (20 μg)
Lane 3 : MCF7 cell lysate (20 μg)
Lane 4 : HepG2 cell lysate (20 μg)
Lanes 1-4 : Merged signal (red and green). Green - ab64533 observed at 20 kDa. Red - loading control ab8245 observed at 37 kDa.
ab64533 Anti-CDC42 antibody was shown to specifically react with CDC42 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266522 (knockout cell lysate ab256868) was used. Wild-type and CDC42 knockout samples were subjected to SDS-PAGE. ab64533 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 μg/ml and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-CDC42 antibody (<a href='/en-us/products/primary-antibodies/cdc42-antibody-ab64533'>ab64533</a>) at 1 µg/mL
Lane 1:
Wild-type HEK-293T cell lysate at 20 µg
Lane 2:
CDC42 knockout HEK-293T cell lysate at 20 µg
Lane 2:
Western blot - Human CDC42 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-cdc42-knockout-hek-293t-cell-line-ab266522'>ab266522</a>)
Lane 3:
MCF7 cell lysate at 20 µg
Lane 4:
HepG2 cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 20 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human CDC42 knockout HEK-293T cell lysate (AB256868)
Homozygous : 2 bp insertion in exon 3
Complementary Products
Primary Antibodies
AB187643
Anti-CDC42 antibody [EPR15620]
RabMAb|Recombinant|KO Validated|20ul selling size
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC42 antibody [EPR15620] (AB187643)](https://content.abcam.com/products/images/cdc42-antibody-epr15620-ab187643--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img92641.jpg)
- 4
- 2
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com