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AB263843

Human CDH2 (N Cadherin) knockout HEK-293T cell lysate

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CDH2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 4 and 5 bp insertion in exon 4.

View Alternative Names

CADH2_HUMAN, CD325, CD325 antigen, CDH2, CDHN, CDw325, CDw325 antigen, Cadherin 2 N cadherin neuronal, Cadherin 2 type 1, Cadherin 2 type 1 N cadherin neuronal, Cadherin 2, type 1, N-cadherin (neuronal), Cadherin-2, Calcium dependent adhesion protein neuronal, N cadherin 1, N-cadherin, NCAD, Neural cadherin, OTTHUMP00000066304, OTTHUMP00000067378

4 Images
Western blot - Human CDH2 (N Cadherin) knockout HEK-293T cell lysate (AB263843)
  • WB

Lab

Western blot - Human CDH2 (N Cadherin) knockout HEK-293T cell lysate (AB263843)

Lane 1 : Wild-type HEK-293T cell lysate (20 μg)

Lane 2 : CDH2 knockout HEK-293T cell lysate (20 μg)

Lanes 1 - 2 : Merged signal (red and green). Green - ab76011 observed at 125 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab76011 was shown to react with N Cadherin in wild-type HEK-293T. Loss of signal was observed when knockout cell line ab255377 (knockout cell lysate ab263843) was used. Wild-type and N Cadherin knockout samples were subjected to SDS-PAGE. ab76011 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 5000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-N Cadherin antibody [EPR1791-4] (<a href='/en-us/products/primary-antibodies/n-cadherin-antibody-epr1791-4-ab76011'>ab76011</a>) at 1/5000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

CDH2 knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human CDH2 (N Cadherin) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-cdh2-n-cadherin-knockout-hek-293t-cell-line-ab255377'>ab255377</a>)

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Predicted band size: 99 kDa

Observed band size: 100 kDa,125 kDa

false

Western blot - Human CDH2 (N Cadherin) knockout HEK-293T cell lysate (AB263843)
  • WB

Lab

Western blot - Human CDH2 (N Cadherin) knockout HEK-293T cell lysate (AB263843)

Lane 1 : Wild-type HEK-293T cell lysate (20 μg)

Lane 2 : CDH2 knockout HEK-293T cell lysate (20 μg)

Lanes 1 - 2 : Merged signal (red and green). Green - ab245117 observed at 125 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab245117 was shown to react with N Cadherin in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab255377 (knockout cell lysate ab263843) was used. Wild-type and N Cadherin knockout samples were subjected to SDS-PAGE. ab245117 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-N Cadherin antibody [EPR22397-264] (<a href='/en-us/products/primary-antibodies/n-cadherin-antibody-epr22397-264-ab245117'>ab245117</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

CDH2 knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human CDH2 (N Cadherin) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-cdh2-n-cadherin-knockout-hek-293t-cell-line-ab255377'>ab255377</a>)

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Predicted band size: 99 kDa

Observed band size: 125 kDa,130 kDa

false

Sanger Sequencing - Human CDH2 (N Cadherin) knockout HEK-293T cell lysate (AB263843)
  • Sanger seq

Unknown

Sanger Sequencing - Human CDH2 (N Cadherin) knockout HEK-293T cell lysate (AB263843)

Allele-2 : 5 bp insertion in exon 4

Sanger Sequencing - Human CDH2 (N Cadherin) knockout HEK-293T cell lysate (AB263843)
  • Sanger seq

Unknown

Sanger Sequencing - Human CDH2 (N Cadherin) knockout HEK-293T cell lysate (AB263843)

Allele-1 : 1 bp insertion in exon 4

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 4 and 5 bp insertion in exon 4.

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Reactivity data

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
CDH2
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

N-Cadherin also known as CDH2 or neuronal cadherin is a calcium-dependent cell adhesion protein. It is characterized by its role in mediating intercellular connections primarily through its presence on neurons fibroblasts and certain epithelial cells. N-Cadherin has an approximate mass of 130 kDa. The protein is integral in forming homophilic interactions where N-Cadherin molecules on adjacent cells interact to establish robust cell-cell adhesion.
Biological function summary

N-Cadherin significantly influences cell-cell interaction and communication facilitating cellular adhesion and signal transduction. It is a vital component of adherens junctions contributing to tissue morphogenesis and stability. N-Cadherin interacts with other cytoplasmic proteins such as catenins forming a complex essential for linking the actin cytoskeleton to the cell membrane. This interaction affects cellular behaviors including migration and differentiation.

Pathways

N-Cadherin plays a significant role in the neural development and epithelial-to-mesenchymal transition (EMT) pathways important for development and cancer progression. It engages with related proteins such as beta-catenin which helps transduce signals within these pathways. N-Cadherin's interactions within these pathways highlight its role in maintaining multicellular structure and signaling processes important for development and pathogenesis.

N-Cadherin is associated with cancer and heart disease. In cancer particularly in the context of the EMT N-Cadherin facilitates tumor progression and metastasis cooperating with proteins like Twist and Snail to promote these processes. In heart disease alterations in N-Cadherin expression and function can impact cardiac muscle integrity and syncytium highlighting potential interactions with connexin43 to maintain cardiac function. Understanding these associations provides insights into therapeutic targets for these conditions.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Complementary Products

Cell Lines & Lysates

AB255377

Human CDH2 (N Cadherin) knockout HEK-293T cell line

Advanced Validation

Immunocytochemistry/ Immunofluorescence - Human CDH2 (N Cadherin) knockout HEK-293T cell line (AB255377)

  • 0
  • 0
Primary Antibodies

AB71916

Anti-EpCAM antibody

BOND RX™ Validated

Immunocytochemistry/ Immunofluorescence - Anti-EpCAM antibody (AB71916)

  • 4
  • 20
Primary Antibodies

AB18203

Anti-N Cadherin antibody - Intercellular Junction Marker

BOND RX™ Validated|Lab Essentials

Western blot - Anti-N Cadherin antibody - Intercellular Junction Marker (AB18203)

  • 4
  • 48
Assay Kits

AB254512

Human N-Cadherin ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human N-Cadherin ELISA Kit (AB254512)

  • 0
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Product promise

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For full details, please see our Terms & Conditions

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