Human CDK17 (PCTAIRE2) knockout HeLa cell lysate
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CDK17 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 2 and 2 bp insertion in exon 2.
View Alternative Names
6430598J10Rik, CDK17_HUMAN, Cell division protein kinase 17, Cyclin-dependent kinase 17, EC 2.7.1.37, MGC25109, PCTAIRE-motif protein kinase 2, PCTK2, Protein kinase cdc2-related PCTAIRE-2, Serine/threonine-protein kinase PCTAIRE-2
- Sanger seq
Unknown
Sanger Sequencing - Human CDK17 (PCTAIRE2) knockout HeLa cell lysate (AB263144)
Allele-1 : 1 bp insertion in exon 2
- Sanger seq
Unknown
Sanger Sequencing - Human CDK17 (PCTAIRE2) knockout HeLa cell lysate (AB263144)
Allele-2 : 2 bp insertion in exon 2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PCTAIRE2 functions in the modulation of neurodevelopmental processes and perhaps in the regulation of signal transmission in neurons. It may form complexes with various regulatory subunits affecting neuronal differentiation and outgrowth specifically in the brain. The presence of PCTAIRE2 in testis tissue indicates its potential role in reproductive biology though this function remains less understood compared to its neurological activities.
Pathways
PCTAIRE2 participates actively in pathways linked to neuronal signaling and development. The phosphorylation activity of PCTAIRE2 intertwines with proteins such as cyclins enabling the progression and regulation of cellular cycles particularly within neuronal cells. Moreover PCTAIRE2 links to pathways that include synaptic signaling interacting with related kinases that impact neuron structure and function though specific relationships to direct pathway members remain under ongoing investigation.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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