Human CDK5 knockout HEK-293T cell lysate
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CDK5 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.
View Alternative Names
CDK5_HUMAN, Cell division protein kinase 5, Crk6, Cyclin-dependent kinase 5, PSSALRE, Protein kinase CDK5 splicing, Serine threonine protein kinase PSSALRE, Serine/threonine-protein kinase PSSALRE, TPKII catalytic subunit, Tau protein kinase II catalytic subunit
- WB
Lab
Western blot - Human CDK5 knockout HEK-293T cell lysate (AB257157)
Lane 1 : Wild-type HEK-293T cell lysate 20 μg
Lane 2 : CDK5 knockout HEK-293T cell lysate 20 μg
Lane 3 : SH-SY5Y cell lysate 20 μg
Lane 4 : MCF7 cell lysate 20 μg
Lanes 1 - 4 : Merged signal (red and green). Green - ab21249 observed at 32 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.
ab21249 was shown to react with Cdk5 in wild-type HEK-293T cells in Western blot with loss of signal observed in CDK5 knockout cell line ab266089 (CDK5 knockout cell lysate ab257157). Wild-type HEK-293T and CDK5 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab21249 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
All lanes:
Anti-CDK5 antibody (<a href='/en-us/products/unavailable/cdk5-antibody-ab21249'>ab21249</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK-293T cell lysate at 20 µg
Lane 2:
CDK5 knockout HEK-293T cell lysate at 20 µg
Lane 2:
Western blot - Human CDK5 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-cdk5-knockout-hek-293t-cell-line-ab266089'>ab266089</a>)
Lane 3:
SH-SY5Y cell lysate at 20 µg
Lane 4:
MCF7 cell lysate at 20 µg
false
- Sanger seq
Unknown
Sanger Sequencing - Human CDK5 knockout HEK-293T cell lysate (AB257157)
Homozygous : 1 bp insertion in exon 1
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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