Human CDK8 knockout HeLa cell lysate
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CDK8 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and 22 bp deletion in exon1.
View Alternative Names
CDK8 protein kinase, CDK8_HUMAN, Cell Division Kinase 8, Cell division protein kinase 8, Cyclin-dependent kinase 8, K35, Mediator complex subunit cdk8, Mediator of RNA polymerase II transcription subunit cdk8, Protein kinase K35
- WB
Lab
Western blot - Human CDK8 knockout HeLa cell lysate (AB257885)
Lane 1 : Wild-type HeLa cell lysate (20 μg)
Lane 2 : CDK8 knockout HeLa cell lysate (20 μg)
Lane 3 : HAP1 cell lysate (20 μg)
Lane 4 : Daudi cell lysate (20 μg)
Lanes 1-4 : Merged signal (red and green). Green - ab229192 observed at 53 kDa. Red - loading control ab8245 observed at 37 kDa.
ab229192 Anti-Cdk8 antibody [EPR21005] was shown to specifically react with Cdk8 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265087 (knockout cell lysate ab257885) was used. Wild-type and Cdk8 knockout samples were subjected to SDS-PAGE. ab229192 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Cdk8 antibody [EPR21005] (<a href='/en-us/products/primary-antibodies/cdk8-antibody-epr21005-ab229192'>ab229192</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
CDK8 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human CDK8 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-cdk8-knockout-hela-cell-line-ab265087'>ab265087</a>)
Lane 3:
HAP1 cell lysate at 20 µg
Lane 4:
Daudi cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution
Predicted band size: 53 kDa
Observed band size: 53 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human CDK8 knockout HeLa cell lysate (AB257885)
Allele-1 : 22 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human CDK8 knockout HeLa cell lysate (AB257885)
Allele-2 : 1 bp insertion in exon1
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Cdk8 plays a critical role in modulating transcriptional responses. It does this by becoming part of the Mediator complex which is essential for transcription regulation by RNA Polymerase II. The Mediator complex where Cdk8 acts as a kinase influences the initiation and regulation of gene expression. Cdk8 phosphorylates several transcription factors and other components affecting their ability to activate or repress transcription.
Pathways
Cyclin-dependent kinase 8 functions prominently in the Wnt/β-catenin signaling and Notch signaling pathways both essential for development and cell differentiation. In the Wnt pathway it interacts closely with proteins like β-catenin to influence gene activation. In the Notch pathway it modulates transcription factors related to cellular differentiation processes. These interactions underline its role as a regulator in carefully managing cellular responses to signaling cues.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
![Immunoprecipitation - Anti-Cdk8 antibody [EPR21005] (AB229192)](https://content.abcam.com/products/images/cdk8-antibody-epr21005-ab229192--immunoprecipitation-img145440.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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