Human CDV3 knockout HEK-293T cell lysate
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CDV3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 3 and Insertion of the selection cassette in exon 3.
View Alternative Names
CDV3 homolog, CDV3 homolog (mouse), CDV3_HUMAN, Carnitine deficiency associated gene expressed in ventricle 3, H41, Protein CDV3 homolog
- Sanger seq
Unknown
Sanger Sequencing - Human CDV3 knockout HEK-293T cell lysate (AB263145)
Allele-1 : 2 bp deletion in exon 3
- Sanger seq
Unknown
Sanger Sequencing - Human CDV3 knockout HEK-293T cell lysate (AB263145)
Allele-2 : Insertion of the selection cassette in exon 3
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CDV3 influences cell death processes particularly apoptosis. It interacts with different effector molecules but does not function as part of a larger protein complex which implies it could exert its biological effects independently. There is evidence it might have a role in regulating non-apoptotic functions such as cell differentiation or stress responses although these functions need further investigation.
Pathways
CDV3 interacts mainly within the apoptosis signaling pathways. It participates in pathways that involve molecules such as BCL-2 family proteins which regulate mitochondrial membrane permeabilization an important step in the apoptotic process. Another associated pathway could involve stress response protein kinases which are related to cell survival and apoptosis although direct interactions require more precise mapping.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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