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AB257388

Human CEACAM1 knockout A549 cell lysate

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CEACAM1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon3 and 2 bp insertion in exon3 and 5 bp deletion in exon3.

View Alternative Names

Antigen CD66, BGP, BGP-1, BGPI, Biliary glycoprotein, Biliary glycoprotein 1, Biliary glycoprotein adhesion molecule, CD66a, CD66a antigen, CEACAM1, CEAM1_HUMAN, Carcinoembryonic antigen-related cell adhesion molecule 1, Meconium antigen 100, carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein)

4 Images
Western blot - Human CEACAM1 knockout A549 cell lysate (AB257388)
  • WB

Unknown

Western blot - Human CEACAM1 knockout A549 cell lysate (AB257388)

Lane 1 : Wild-type A549 cell lysate (20 ug)
Lane 2 : CEACAM1 knockout A549 cell lysate (20 ug)
Lane 3 : A549 cell lysate (20 ug)

ab108397 was shown to specifically react with CEACAM1 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267046 (knockout cell lysate ab257388) was used. Wild-type and CEACAM1 knockout samples were subjected to SDS-PAGE. ab108397 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

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Sanger Sequencing - Human CEACAM1 knockout A549 cell lysate (AB257388)
  • Sanger seq

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Sanger Sequencing - Human CEACAM1 knockout A549 cell lysate (AB257388)

Allele-1 : 5 bp deletion in exon3

Sanger Sequencing - Human CEACAM1 knockout A549 cell lysate (AB257388)
  • Sanger seq

Unknown

Sanger Sequencing - Human CEACAM1 knockout A549 cell lysate (AB257388)

Allele-3 : 2 bp insertion in exon3

Sanger Sequencing - Human CEACAM1 knockout A549 cell lysate (AB257388)
  • Sanger seq

Unknown

Sanger Sequencing - Human CEACAM1 knockout A549 cell lysate (AB257388)

Allele-2 : 1 bp insertion in exon3

Key facts

Cell type

A549

Species or organism

Human

Tissue

Lung

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon3 and 2 bp insertion in exon3 and 5 bp deletion in exon3.

Disease

Carcinoma

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
CEACAM1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CEACAM1 also known as CD66a is a cell adhesion molecule that plays a critical role in various cellular processes. The protein weighs around 120 kDa and is part of the immunoglobulin superfamily. CEACAM1 is expressed on the surface of various cell types including epithelial cells leukocytes and endothelial cells. The presence of CEACAM1 is important in tissues such as the liver lungs and gastrointestinal tract where it performs essential roles in cell signaling and communication.
Biological function summary

CEACAM1 influences cellular adhesion and immune responses by mediating cell-cell interactions. It forms homophilic dimers at the cell surface and it can participate in heterophilic interactions with other proteins making it part of complex cell signaling networks. These interactions regulate immune cell functions contributing to innate immune responses and cellular homeostasis. CEACAM1 also modulates angiogenesis endothelial cell permeability and apoptosis.

Pathways

CEACAM1 is involved in signaling and regulatory pathways important for immune surveillance and inflammation. It is a part of the TGF-beta signaling pathway affecting cellular proliferation and differentiation. CEACAM1 interacts with proteins such as SHP-1 and SHP-2 which are essential in modulating immune responses. Additionally CEACAM1 influences the MAPK/ERK pathway which plays a role in cell growth and survival.

CEACAM1 is associated with cancer and inflammatory diseases. In colorectal cancer altered expression of CEACAM1 contributes to tumor progression and metastasis. The protein also plays a role in inflammatory bowel disease where it affects the epithelial barrier function and immune cell interaction. Through these conditions CEACAM1 interacts with other proteins like CEA and IL-6 which are pivotal in disease development and progression.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Male

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Product protocols

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Product promise

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For full details, please see our Terms & Conditions

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