Human CEBPB (CEBP Beta) knockout HeLa cell lysate
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CEBPB KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon1 and 1 bp deletion in exon1.
View Alternative Names
AGP/EBP, C EBP beta, C/EBP beta, C/EBP related protein 2, CCAAT Enhancer Binding Protein beta, CCAAT/enhancer-binding protein beta, CEBPB_HUMAN, CRP2, IL 6DBP, Interleukin 6 dependent binding protein, LAP, Liver activator protein, Liver enriched transcriptional activator, NF IL6, Nuclear factor NF-IL6, SF B, Silencer factor B, TCF-5, Transcription factor 5
- WB
Lab
Western blot - Human CEBPB (CEBP Beta) knockout HeLa cell lysate (AB256874)
Lane 1 : Wild-type HeLa cell lysate (20 μg)
Lane 2 : CEBPB knockout HeLa cell lysate (20 μg)
Lane 3 : Jurkat cell lysate (20 μg)
Lanes 1-3 : Merged signal (red and green). Green - ab32358 observed at 40 and 45 kDa. Red - loading control ab7291 observed at 50 kDa.
ab32358 Anti-CEBP Beta antibody [E299] - C-terminal was shown to specifically react with CEBP Beta in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261771 (knockout cell lysate ab256874) was used. Wild-type and CEBP Beta knockout samples were subjected to SDS-PAGE. ab32358 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-CEBP Beta antibody [E299] - C-terminal (<a href='/en-us/products/primary-antibodies/cebp-beta-antibody-e299-c-terminal-ab32358'>ab32358</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
CEBP Beta knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human CEBPB (CEBP Beta) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-cebpb-cebp-beta-knockout-hela-cell-line-ab261771'>ab261771</a>)
Lane 3:
Jurkat cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 40 kDa,45 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human CEBPB (CEBP Beta) knockout HeLa cell lysate (AB256874)
Allele-2 : 1 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human CEBPB (CEBP Beta) knockout HeLa cell lysate (AB256874)
Allele-1 : 11 bp deletion in exon1
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CEBP Beta plays important roles in cellular differentiation proliferation and immune function. CEBP Beta is part of the CEBP family of transcription factors which form homo- or hetero-dimers to function effectively often with other family members like CEBP Alpha. Its activity governs important processes in hematopoiesis and adipogenesis. This transcription factor is therefore essential in the regulation of energy homeostasis and immune response influencing the expression of various cytokines and acute-phase proteins.
Pathways
CEBP Beta integrates signals in key biological pathways such as the JAK-STAT and NF-kB pathways. Activation via cytokine signaling CEBP Beta interacts with STAT proteins to modulate gene expression linked to inflammatory responses. It has a significant role in the NF-kB pathway where it cooperates with Rel proteins to regulate genes involved in immune and stress responses including TNF and IL-1.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB32358
Anti-CEBP Beta antibody [E299] - C-terminal
RabMAb|Recombinant|KO Validated|20ul selling size
![Flow Cytometry (Intracellular) - Anti-CEBP Beta antibody [E299] - C-terminal (AB32358)](https://content.abcam.com/products/images/cebp-beta-antibody-e299-c-terminal-ab32358--flow-cytometry-intracellular-img89243.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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