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AB257888

Human CECR5 knockout HEK-293T cell lysate

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HDHD5 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 17 bp deletion in exon 6 and 1 bp deletion in exon 6.

View Alternative Names

A930002G03Rik, CECR5_HUMAN, Cat eye syndrome chromosome region, candidate 5, Cat eye syndrome chromosome region, candidate 5 homolog, Cat eye syndrome critical region protein 5, MGC25951, OTTMUSP00000025905

3 Images
Western blot - Human CECR5 knockout HEK-293T cell lysate (AB257888)
  • WB

Unknown

Western blot - Human CECR5 knockout HEK-293T cell lysate (AB257888)

Lane 1 : Wild-type HEK293T cell lysate (20 ug)
Lane 2 : CECR5 knockout HEK293T cell lysate (20 ug)
Lane 3 : HeLa cell lysate (20 ug)
Lane 4 : Jurkat cell lysate (20 ug)

ab184185 was shown to specifically react with CECR5 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266708 (knockout cell lysate ab257888) was used. Wild-type and CECR5 knockout samples were subjected to SDS-PAGE. ab184185 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

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Sanger Sequencing - Human CECR5 knockout HEK-293T cell lysate (AB257888)
  • Sanger seq

Unknown

Sanger Sequencing - Human CECR5 knockout HEK-293T cell lysate (AB257888)

Allele-2 : 1 bp deletion in exon 6

Sanger Sequencing - Human CECR5 knockout HEK-293T cell lysate (AB257888)
  • Sanger seq

Unknown

Sanger Sequencing - Human CECR5 knockout HEK-293T cell lysate (AB257888)

Allele-1 : 17 bp deletion in exon 6

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 17 bp deletion in exon 6 and 1 bp deletion in exon 6.

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Reactivity data

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
HDHD5
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CECR5 also known as Cat Eye Syndrome Critical Region Candidate 5 encodes a protein with a mass of about 48 kDa. This protein shows expression in multiple tissues such as the brain kidney and liver. Its mechanical role involves binding interactions that help in signaling and cellular communication processes. The protein's structure enables it to participate effectively in these interactions making it a point of interest for further study in these specific tissues.
Biological function summary

CECR5 serves an important function in cellular signaling networks and is not typically found as part of a larger complex. The protein plays a role in modulating certain cellular responses possibly impacting processes like differentiation and growth. Researchers have identified CECR5 involvement in cellular mechanisms that govern normal development and maintenance hinting at its broader influence in cellular homeostasis.

Pathways

CECR5 engages in the MAPK signaling pathway which is instrumental in transferring extracellular signals to intracellular responses. The MAPK pathway involves proteins like ERK and JNK with which CECR5 may interact or have regulatory effects. Additionally it influences signaling via the PI3K-AKT pathway a pathway that controls many aspects of cell survival growth and metabolism. This association suggests CECR5's potential roles in important cellular activities and regulatory mechanisms.

Alterations in CECR5 expression or function relate to Cat Eye Syndrome and possibly neurodevelopmental disorders. In Cat Eye Syndrome the overexpression or structural changes of CECR5 may disrupt normal development alongside chromosomal duplications involving the region. Connections also appear between CECR5 and other proteins like PAX6 in the context of its role in developmental processes. Understanding these relationships further explains CECR5's impact on these conditions.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

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