Human CERS6 (LASS6) knockout HEK-293T cell lysate
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CERS6 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.
View Alternative Names
Ceramide synthase 6, LAG1 homolog ceramide synthase 6, LAG1 longevity assurance homolog 6, LASS 6, LASS6_HUMAN, Longevity assurance homolog 6, MGC129949, MGC129950, T1L
- Sanger seq
Unknown
Sanger Sequencing - Human CERS6 (LASS6) knockout HEK-293T cell lysate (AB258354)
Homozygous : 1 bp insertion in exon 1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CERS6 is involved in the synthesis of longer-chain ceramides which impacts cell membrane structure and signaling. CERS6 performs its function alone and not as part of a larger complex. The enzyme's activity influences cellular processes such as proliferation differentiation and apoptosis contributing to cellular homeostasis. Ceramides produced by CERS6 affect membrane properties and have roles in signal transduction pathways.
Pathways
CERS6 is an important player in the sphingolipid metabolism pathway affecting lipid signaling and apoptosis regulation. This enzyme collaborates with other ceramide synthases such as CERS2 in maintaining balance in ceramide levels influencing cell fate decisions. It also participates in interconnected pathways like the endoplasmic reticulum stress response interacting with proteins such as PERK to modulate apoptosis under stress conditions.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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