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AB257150

Human CFH (Factor H) knockout A549 cell lysate

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CFH KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon3 and 1 bp deletion in exon3 and 2 bp deletion in exon3.
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Western blot - Human CFH (Factor H) knockout A549 cell lysate (AB257150)
  • WB

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Western blot - Human CFH (Factor H) knockout A549 cell lysate (AB257150)

Lane 1 : Wild-type A549 cell lysate (20 ug)
Lane 2 : CFH knockout A549 cell lysate (20 ug)
Lane 3 : HaCaT cell lysate (20 ug)

ab133536 was shown to specifically react with Factor H in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267031 (knockout cell lysate ab257150) was used. Wild-type and Factor H knockout samples were subjected to SDS-PAGE. ab133536 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

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Sanger Sequencing - Human CFH (Factor H) knockout A549 cell lysate (AB257150)
  • Sanger seq

Unknown

Sanger Sequencing - Human CFH (Factor H) knockout A549 cell lysate (AB257150)

Allele-2 : 2 bp deletion in exon3

Sanger Sequencing - Human CFH (Factor H) knockout A549 cell lysate (AB257150)
  • Sanger seq

Unknown

Sanger Sequencing - Human CFH (Factor H) knockout A549 cell lysate (AB257150)

Allele-3 : 1 bp deletion in exon3

Sanger Sequencing - Human CFH (Factor H) knockout A549 cell lysate (AB257150)
  • Sanger seq

Unknown

Sanger Sequencing - Human CFH (Factor H) knockout A549 cell lysate (AB257150)

Allele-1 : 10 bp deletion in exon3

Key facts

Cell type

A549

Species or organism

Human

Tissue

Lung

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon3 and 1 bp deletion in exon3 and 2 bp deletion in exon3.

Disease

Carcinoma

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Cell Lines & Lysates

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Primary Antibodies

AB118820

Anti-Factor H antibody [OX-24]

Trial Size

ELISA - Anti-Factor H antibody [OX-24] (AB118820)

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Assay Kits

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Human Complement factor H ELISA Kit

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ELISA - Human Complement factor H ELISA Kit (AB252359)

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Reactivity data

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
CFH
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor H also known as complement factor H is an important regulatory protein in the complement system. It has a molecular mass of approximately 155 kDa. This protein is mainly expressed in the liver but it can also be found in low levels in other tissues. Factor H serves as a control element for complement activation particularly affecting the alternative pathway. It binds to C3b a central component of the complement system and accelerates the decay of C3 convertase as well as promotes the proteolytic inactivation of C3b by factor I.
Biological function summary

Factor H limits the activity of the complement system to prevent damage to host tissues. The protein exists in the plasma in a soluble form. It functions by recognizing host cell surfaces via specific markers avoiding inappropriate activation. Factor H belongs to a group of proteins which include other regulators of complement activation. These proteins maintain the balance between effective immune defense and protection of host tissue from excessive immune responses.

Pathways

Factor H is a part of the alternative complement pathway. This pathway is important for innate immune response involving proteins like factor P (properdin) which stabilizes C3 convertase. Factor H modulates these interactions to prevent unwarranted complement activity on host cells. Another related pathway is the classic complement pathway although factor H's involvement here is less direct since it primarily regulates the alternative pathway.

Factor H associations include atypical hemolytic uremic syndrome and age-related macular degeneration. Factor H deficiency or dysfunction can lead to uncontrolled complement activation resulting in kidney damage in atypical hemolytic uremic syndrome where it is also related to factor I. Additionally in age-related macular degeneration variants in the factor H gene are linked to increased susceptibility further highlighting the protein's importance in regulating immune responses.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Male

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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