Human CHCHD6 knockout HEK-293T cell lysate
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CHCHD6 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon1.
View Alternative Names
CHCH6_HUMAN, CHCM1, Coiled coil helix cristae morphology 1, Coiled coil helix cristae morphology protein 1, Coiled-coil-helix-coiled-coil-helix domain-containing protein 6, Coiled-coil-helix-coiled-coil-helix domain-containing protein 6, mitochondrial, MICOS complex subunit MIC25, PPP1R23, Protein phosphatase 1 regulatory subunit 23
- Sanger seq
Unknown
Sanger Sequencing - Human CHCHD6 knockout HEK-293T cell lysate (AB263150)
Allele-2 : Insertion of the selection cassette in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human CHCHD6 knockout HEK-293T cell lysate (AB263150)
Allele-1 : Insertion of the selection cassette in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The coiled-coil-helix domain containing protein 6 is involved in mitochondrial function and maintenance. CHCHD6 participates in the formation of complexes within the inner mitochondrial membrane. These complexes are critical for the proper assembly and stability of mitochondrial respiratory supercomplexes which facilitate efficient electron transport and energy production. This involvement positions CHCHD6 as an important player in maintaining cellular energy dynamics and bioenergetics.
Pathways
CHCHD6 has a role in the mitochondrial electron transport chain which is important for ATP production. This protein associates with pathways governing mitochondrial dynamics and energy metabolism. CHCHD6 interacts with mitochondrial proteins like OPA1 which regulates mitochondrial fusion and cristae morphology contributing to the maintenance of respiratory chain integrity and energy output.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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