Human CHEK2 (Chk2) knockout HeLa cell lysate
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CHEK2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 5.
View Alternative Names
CHEK 2, CHK2 checkpoint homolog, CHK2 checkpoint homolog (S. pombe), CHK2_HUMAN, Cds1 homolog, Checkpoint kinase 2, Checkpoint like protein CHK2, HuCds 1, LFS 2, PP1425, Rad53 homolog, Serine/threonine-protein kinase Chk2, hCds1
- WB
Lab
Western blot - Human CHEK2 (Chk2) knockout HeLa cell lysate (AB257104)
Lane 1 : Wild-type HeLa cell lysate (20 μg)
Lane 2 : CHEK2 knockout HeLa cell lysate (20 μg)
Lane 3 : HEK-293 cell lysate (20 μg)
Lane 4 : MDA-MB-231 cell lysate (20 μg)
Lanes 1-4 : Merged signal (red and green). Green - ab109413 observed at 68 kDa. Red - loading control ab8245 observed at 37 kDa.
ab109413 Anti-Chk2 antibody [EPR4325] was shown to specifically react with Chk2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264815 (knockout cell lysate ab257104) was used. Wild-type and Chk2 knockout samples were subjected to SDS-PAGE. ab109413 and Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab52866) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Chk2 antibody [EPR4325] (<a href='/en-us/products/primary-antibodies/chk2-antibody-epr4325-ab109413'>ab109413</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
CHEK2 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human CHEK2 (Chk2) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-chek2-chk2-knockout-hela-cell-line-ab264815'>ab264815</a>)
Lane 3:
HEK-293 cell lysate at 20 µg
Lane 4:
MDA-MB-231 cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution
Predicted band size: 61 kDa
Observed band size: 68 kDa
false
- WB
Lab
Western blot - Human CHEK2 (Chk2) knockout HeLa cell lysate (AB257104)
Lane 1 : Wild-type HeLa cell lysate (20 μg)
Lane 2 : CHEK2 knockout HeLa cell lysate (20 μg)
Lane 3 : HEK-293 cell lysate (20 μg)
Lane 4 : MDA-MB-231 cell lysate (20 μg)
Lanes 1-4 : Merged signal (red and green). Green - ab207446 observed at 68 kDa. Red - loading control ab8245 observed at 37 kDa.
ab207446 Anti-Chk2 antibody [EPR19482] was shown to specifically react with Chk2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264815 (knockout cell lysate ab257104) was used. Wild-type and Chk2 knockout samples were subjected to SDS-PAGE. ab207446 and Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab52866) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Chk2 antibody [EPR19482] (<a href='/en-us/products/primary-antibodies/chk2-antibody-epr19482-ab207446'>ab207446</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
CHEK2 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human CHEK2 (Chk2) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-chek2-chk2-knockout-hela-cell-line-ab264815'>ab264815</a>)
Lane 3:
HEK-293 cell lysate at 20 µg
Lane 4:
MDA-MB-231 cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution
Predicted band size: 61 kDa
Observed band size: 68 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human CHEK2 (Chk2) knockout HeLa cell lysate (AB257104)
Homozygous : 1 bp insertion in exon 5
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB109413
20ul selling size|RabMAb|Recombinant|KO Validated
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chk2 antibody [EPR4325] (AB109413)](https://content.abcam.com/products/images/chk2-antibody-epr4325-ab109413--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img91668.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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