Human CHI3L1 knockout THP-1 cell lysate
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CHI3L1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 14 bp deletion in exon 7.
View Alternative Names
39 kDa synovial protein, ASRT7, CGP-39, CH3L1_HUMAN, CHI3L1, Cartilage glycoprotein 39, Chitinase 3 like protein 1 precursor, Chitinase-3-like protein 1, Chondrocyte protein YKL40, GP-39, HCGP 3P, YKL-40, YYL 40, chitinase, chitinase 3 like 1, chitinase 3 like 1 (cartilage glycoprotein 39), hCGP-39
- WB
Lab
Western blot - Human CHI3L1 knockout THP-1 cell lysate (AB280097)
Lane 1 : Wild-type THP-1 cell lysate 20 μg
Lane 2 : CHI3L1 knockout THP-1 cell lysate 20 μg
Lane 3 : U-87 MG cell lysate 20 μg
Lane 4 : Jurkat cell lysate 20 μg
False colour image of Western blot : Anti-YKL-40/CHI3L1 antibody staining at 1 μg/ml, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab77528 was shown to bind specifically to YKL-40/CHI3L1. A band was observed at 33-42 kDa in wild-type THP-1 cell lysates with no signal observed at this size in CHI3L1 knockout cell line ab280038 (knockout cell lysate ab280097). To generate this image, wild-type and CHI3L1 knockout THP-1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-YKL-40/CHI3L1 antibody (<a href='/en-us/products/primary-antibodies/ykl-40-chi3l1-antibody-ab77528'>ab77528</a>) at 1 µg/mL
Lane 1:
Wild-type THP-1 cell lysate at 20 µg
Lane 2:
CHI3L1 knockout THP-1 cell lysate at 20 µg
Lane 2:
Western blot - Human CHI3L1 knockout THP-1 cell line (<a href='/en-us/products/cell-lines/human-chi3l1-knockout-thp-1-cell-line-ab280038'>ab280038</a>)
Lane 3:
U-87 MG cell lysate at 20 µg
Lane 4:
Jurkat cell lysate at 20 µg
Predicted band size: 43 kDa
Observed band size: 33-42 kDa
false
- Sanger seq
Supplier Data
Sanger Sequencing - Human CHI3L1 knockout THP-1 cell lysate (AB280097)
14 bp deletion in exon 7
- WB
Lab
Western blot - Human CHI3L1 knockout THP-1 cell lysate (AB280097)
Lane 1 : Wild-type THP-1 cell lysate 20 μg
Lane 2 : CHI3L1 knockout THP-1 cell lysate 20 μg
Lane 3 : U-87 MG cell lysate 20 μg
Lane 4 : Jurkat cell lysate 20 μg
False colour image of Western blot : Anti-YKL-40/CHI3L1 antibody [EPR19078-157] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab255297 was shown to bind specifically to YKL-40/CHI3L1. A band was observed at 37/40 kDa in wild-type THP-1 cell lysates with no signal observed at this size in CHI3L1 knockout cell line ab280038 (knockout cell lysate ab280097). To generate this image, wild-type and CHI3L1 knockout THP-1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-YKL-40/CHI3L1 antibody [EPR19078-157] (<a href='/en-us/products/primary-antibodies/ykl-40-chi3l1-antibody-epr19078-157-ab255297'>ab255297</a>) at 1/1000 dilution
Lane 1:
Wild-type THP-1 cell lysate at 20 µg
Lane 2:
Western blot - Human CHI3L1 knockout THP-1 cell lysate (ab280097) at 20 µg
Lane 3:
U-87 MG cell lysate at 20 µg
Lane 4:
Jurkat cell lysate at 20 µg
Secondary
Lanes 1 - 4:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution
Lanes 1 - 4:
Western blot - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>) at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 37 kDa,40 kDa,55 kDa
false
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
YKL-40 plays significant roles in tissue remodeling and inflammation. It does not form part of a larger complex by itself but influences several cellular processes. This protein affects cell proliferation migration and survival making it involved in both normal physiological processes and pathological conditions. CHI3L1 also exhibits a connection to the immune response where it modulates macrophage activity and interacts with other immune cells.
Pathways
YKL-40 is integral to the inflammatory and extracellular matrix pathways. It associates with proteins such as interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) influencing signaling cascades that modulate inflammation. Through these interactions CHI3L1 becomes a mediator of connective tissue metabolism and immune reactions in various pathophysiological states.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Suspension
Gender
Male
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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