Human CIRBP (CIRP) knockout HEK-293T cell lysate
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- WB
Lab
Western blot - Human CIRBP (CIRP) knockout HEK-293T cell lysate (AB257252)
Lane 1 : Wild-type HEK-293T cell lysate (20 μg)
Lane 2 : CIRBP knockout HEK-293T cell lysate (20 μg)
Lanes 1-2 : Merged signal (red and green). Green - ab191885 observed at 19 kDa. Red - loading control ab8245 observed at 37 kDa.
ab191885 Anti-CIRP antibody [EPR18783] was shown to specifically react with CIRP in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266187 (knockout cell lysate ab257252) was used. Wild-type and CIRP knockout samples were subjected to SDS-PAGE. ab191885 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-CIRP antibody [EPR18783] (<a href='/en-us/products/primary-antibodies/cirp-antibody-epr18783-ab191885'>ab191885</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK-293T cell lysate at 20 µg
Lane 2:
CIRP knockout HEK-293T cell lysate at 20 µg
Lane 2:
Western blot - Human CIRBP (CIRP) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-cirbp-cirp-knockout-hek-293t-cell-line-ab266187'>ab266187</a>)
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution
Predicted band size: 18 kDa
Observed band size: 19 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human CIRBP (CIRP) knockout HEK-293T cell lysate (AB257252)
Homozygous : 7 bp deletion in exon2
Complementary Products
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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