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AB261698

Human CLDN1 knockout A-431 cell lysate

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CLDN1 KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9; X = 5 bp deletion, 2 bp deletion; Frameshift = 99.7%.
4 Images
Western blot - Human CLDN1 knockout A-431 cell lysate (AB261698)
  • WB

Lab

Western blot - Human CLDN1 knockout A-431 cell lysate (AB261698)

Lane 1 : Wild-type A-431 (Human epidermoid carcinoma cell line) whole cell lysate 20 ug
Lane 2 : CLDN1 knockout A-431 (Human epidermoid carcinoma cell line) whole cell lysate 20 ug
Lane 3 : A549 (Human lung carcinoma cell line) whole cell lysate 20 ug
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate 20 ug
Lanes 1 - 4 : Merged signal (red and green). Green - ab211737 observed at 18 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab211737 was shown to specifically react with in wild-type A-431 WT cells as signal was lost in CLDN1 knockout A-431 cell line ab261889 (knockout cell lysate ab261698). Wild-type and CLDN1 knockout A-431 samples were subjected to SDS-PAGE. The membrane was blocked with 3% milk. ab211737 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Claudin 1 antibody [EPRR18871] (<a href='/en-us/products/primary-antibodies/claudin-1-antibody-eprr18871-ab211737'>ab211737</a>) at 1/2000 dilution

Lane 1:

Wild-type A-431 (Human epidermoid carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

CLDN1 knockout A-431 (Human epidermoid carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

Western blot - Human CLDN1 knockout A-431 cell line (<a href='/en-us/products/cell-lines/human-cldn1-knockout-a-431-cell-line-ab261889'>ab261889</a>)

Lane 3:

A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 4:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Predicted band size: 22 kDa

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Western blot - Human CLDN1 knockout A-431 cell lysate (AB261698)
  • WB

Lab

Western blot - Human CLDN1 knockout A-431 cell lysate (AB261698)

Lane 1 : Wild-type A-431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : CLDN1 knockout A-431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : A549 (Human lung carcinoma cell line) whole cell lysate
Lanes 1 - 3 : Merged signal (red and green). Green - ab180158 observed at 18 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab180158 was shown to specifically react with in wild-type A-431 cells as signal was lost in CLDN1 knockout A-431 cell line ab261889 (knockout cell lysate ab261698). Wild-type and A-431 CLDN1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% milk. ab180158 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Claudin 1 antibody [EPR9306] (<a href='/en-us/products/primary-antibodies/claudin-1-antibody-epr9306-ab180158'>ab180158</a>) at 1/10000 dilution

Lane 1:

Wild-type A-431 (Human epidermoid carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

CLDN1 knockout A-431 (Human epidermoid carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

Western blot - Human CLDN1 knockout A-431 cell line (<a href='/en-us/products/cell-lines/human-cldn1-knockout-a-431-cell-line-ab261889'>ab261889</a>)

Lane 3:

A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Predicted band size: 22 kDa

false

Western blot - Human CLDN1 knockout A-431 cell lysate (AB261698)
  • WB

Lab

Western blot - Human CLDN1 knockout A-431 cell lysate (AB261698)

Lane 1 : Wild-type A-431 (Human epidermoid carcinoma cell line) whole cell lysate

Lane 2 : CLDN1 knockout A-431 (Human epidermoid carcinoma cell line) whole cell lysate

Lanes 1 - 2 : Merged signal (red and green). Green - ab129119 observed at 18 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab129119 was shown to specifically react with in wild-type A-431 cells as signal was lost in CLDN1 knockout cell line ab261889 (knockout cell lysate ab261698). Wild-type and CLDN1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% milk. ab129119 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Claudin 1 antibody (<a href='/en-us/products/primary-antibodies/claudin-1-antibody-ab129119'>ab129119</a>) at 1 µg/mL

Lane 1:

Wild-type A-431 (Human epidermoid carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

CLDN1 knockout A-431 (Human epidermoid carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

Western blot - Human CLDN1 knockout A-431 cell line (<a href='/en-us/products/cell-lines/human-cldn1-knockout-a-431-cell-line-ab261889'>ab261889</a>)

Predicted band size: 22 kDa

Observed band size: 18 kDa

false

Next Generation Sequencing - Human CLDN1 knockout A-431 cell lysate (AB261698)
  • NGS

Lab

Next Generation Sequencing - Human CLDN1 knockout A-431 cell lysate (AB261698)

X = 5 bp deletion, 2 bp deletion

Key facts

Cell type

A-431

Species or organism

Human

Tissue

Skin

Knockout validation

Next Generation Sequencing,Western blot

Mutation description

Knockout achieved by CRISPR/Cas9; X = 5 bp deletion, 2 bp deletion; Frameshift = 99.7%

Disease

Epidermoid Carcinoma

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Complementary Products

Cell Lines & Lysates

AB261889

Human CLDN1 knockout A-431 cell line

Advanced Validation

Western blot - Human CLDN1 knockout A-431 cell line (AB261889)

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  • 0
Primary Antibodies

AB15098

Anti-Claudin 1 antibody

Western blot - Anti-Claudin 1 antibody (AB15098)

  • 4
  • 21
Primary Antibodies

AB216327

Anti-Occludin antibody [EPR20992]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] (AB216327)

  • 4
  • 7
Primary Antibodies

AB221547

Anti-ZO1 tight junction protein antibody [EPR19945-296]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-296] (AB221547)

  • 5
  • 6
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Reactivity data

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab261698-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human CLDN1 knockout A431 cell lysate", "number":"AB261698-CMP02", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type A-431 cell lysate", "number":"AB261698-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Gene name
CLDN1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Next Generation Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Claudin 1 also known as CLDN1 is a protein that plays a fundamental role in the formation of tight junctions in the epithelial cells. This claudin protein has a molecular weight of approximately 22 kDa. You can find it expressed in various tissues including the skin liver kidney and lungs. Claudin 1 belongs to the claudin family which consists of transmembrane proteins involved in sealing the space between adjacent cells therefore ensuring tissue integrity.
Biological function summary

This claudin protein contributes to establishing and maintaining the barrier function that controls paracellular transport which is the movement of substances between cells. Claudin 1 participates as a critical component of the tight junction complex and it interacts with other proteins such as occludin and zonula occludens. These interactions contribute to the regulation and maintenance of cell polarity and signaling.

Pathways

Claudin 1 is involved in the epithelial cell signaling pathways that influence cellular proliferation and differentiation. One significant pathway is the Wnt signaling pathway where claudin 1 cooperates with other claudins and proteins like β-catenin to regulate gene transcription. Additionally it is implicated in the MAPK pathway which involves signaling cascades that affect cell growth division and response to external stress.

Claudin 1 shows a strong association with cancers particularly hepatocellular carcinoma and melanoma. In these conditions changes in claudin 1 expression levels can affect tumor progression and metastasis. It also associates with other proteins like E-cadherin impacting cell adhesion and epithelial-mesenchymal transition in cancerous cells. Moreover abnormalities in claudin 1 expression relate to skin disorders such as ichthyosis which is marked by a dysfunction in the skin barrier.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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