AB258366

Human CLTA (Clathrin light chain A) knockout HEK-293T cell lysate

Name: Human CLTA (Clathrin light chain A) knockout HEK-293T cell lysate (ab258366) | Abcam
Brand: Abcam Limited
SKU: AB258366
Price: 675 USD
Availability: InStock

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CLTA KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 2 bp deletion in exon1.

View Alternative Names

CLCA_HUMAN, CLTA, CLTB, Clathrin light chain A, Clathrin light chain B, Clathrin light chain LCA, Clathrin light chain LCB, Clathrin light polypeptide, Clathrin light polypeptide A, Clathrin light polypeptide B, Clathrin light polypeptide LCA, Clathrin light polypeptide LCB, Clathrin, light chain (Lcb), Clathrin, light polypeptide (Lca), Clathrin, light polypeptide (Lcb), L-CA, LCB

3 Images

Lab

Western blot - Human CLTA (Clathrin light chain A) knockout HEK-293T cell lysate (AB258366)

Lane 1 : Wild-type HEK-293T cell lysate, 20 ug

Lane 2 : CLTA knockout HEK-293T cell lysate, 20 ug

Lane 3 : A431 cell lysate, 20 ug

Lane 4 : HeLa cell lysate, 20 ug

False colour image of Western blot : Anti-Clathrin light chain antibody [EPR24231-72] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab271185 was shown to bind specifically to Clathrin light chain. A band was observed at 35 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in CLTA knockout cell line ab267334 (knockout cell lysate ab258366). To generate this image, wild-type and CLTA knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

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Sanger Sequencing - Human CLTA (Clathrin light chain A) knockout HEK-293T cell lysate (AB258366)

Sanger seq

Unknown

Sanger Sequencing - Human CLTA (Clathrin light chain A) knockout HEK-293T cell lysate (AB258366)

Allele-2 : 1 bp deletion in exon1

Sanger seq

Unknown

Sanger Sequencing - Human CLTA (Clathrin light chain A) knockout HEK-293T cell lysate (AB258366)

Allele-1 : 2 bp deletion in exon1

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 2 bp deletion in exon1.

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name

CLTA

Gene editing type

Knockout

Gene editing method

CRISPR technology

Knockout validation

Sanger Sequencing

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Clathrin light chain also known as clathrin LCa and LCb is a low-molecular-weight component of the clathrin protein complex. It plays a mechanical role in the processes that drive clathrin-mediated endocytosis. Each clathrin triskelion the basic unit of the clathrin coat comprises three clathrin heavy chains and three clathrin light chains. The mass of clathrin light chain is approximately 25-29 kDa. This protein is expressed in various tissues throughout the body with notable presence in the central nervous system and liver.

Biological function summary

Clathrin light chain modulates the lattice dynamics by interacting with clathrin heavy chains in a clathrin protein complex. This interaction supports the stability and assembly of the clathrin lattice affecting the efficiency and specificity of endocytic vesicle formation. Clathrin light chain acts in concert with other accessory proteins to regulate clathrin coat assembly and disassembly playing a dynamic role in cellular trafficking processes.

Pathways

The clathrin light chain participates in essential cellular pathways such as the clathrin-mediated endocytosis and synaptic vesicle recycling pathways. These pathways facilitate the internalization and recycling of membrane proteins and lipids ensuring cellular homeostasis. In these processes the clathrin light chain works closely with related proteins such as dynamin and adaptin to control vesicle scission and cargo selection.

Abnormalities in clathrin light chain function connect to neurodegenerative disorders like Huntington's disease and certain types of cancers. In Huntington's disease disrupted endocytic pathways that involve clathrin light chain impact cellular communication and nutrient uptake. Cancer cells may exhibit altered expression or function of clathrin light chain affecting pathways important for cell proliferation and survival. The clathrin light chain can interact with the huntingtin protein in these contexts influencing disease progression and cellular dynamics.

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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human CLTA (Clathrin light chain A) knockout HEK-293T cell lysate

Western blot - Human CLTA (Clathrin light chain A) knockout HEK-293T cell lysate (AB258366)

Sanger Sequencing - Human CLTA (Clathrin light chain A) knockout HEK-293T cell lysate (AB258366)

Sanger Sequencing - Human CLTA (Clathrin light chain A) knockout HEK-293T cell lysate (AB258366)

Key facts

Cell type

Species or organism

Tissue

Knockout validation

Mutation description

Product details

What's included?

Properties and storage information

Gene name

Gene editing type

Gene editing method

Knockout validation

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Quality control

STR analysis

Cell culture

Biosafety level

Adherent/suspension

Gender

Product protocols

Complementary Products

Cell Lines & Lysates

Primary Antibodies

Primary Antibodies

Proteins & Peptides

Product promise