Human COIL (Coilin) knockout HeLa cell lysate
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COIL KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 10 bp deletion in exon 1.
View Alternative Names
CLN 80, COIL_HUMAN, Coilin, Coilin p80, P80 coilin, p80
- WB
Unknown
Western blot - Human COIL (Coilin) knockout HeLa cell lysate (AB257251)
Lane 1 : Wild-type HeLa cell lysate (20 μg)
Lane 2 : COIL knockout HeLa cell lysate (20 μg)
Lane 3 : Jurkat cell lysate (20 μg)
Lanes 1-3 : Merged signal (red and green). Green - ab87913 observed at 75 kDa. Red - loading control, ab181602 observed at 37 kDa.
ab87913 Anti-Coilin antibody [IH10] was shown to specifically react with Coilin in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261757 (knockout cell lysate ab257251) was used. Wild-type and Coilin knockout samples were subjected to SDS-PAGE. ab87913 and Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Coilin antibody [IH10] (<a href='/en-us/products/primary-antibodies/coilin-antibody-ih10-ab87913'>ab87913</a>) at 1/500 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
COIL knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human COIL (Coilin) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-coil-coilin-knockout-hela-cell-line-ab261757'>ab261757</a>)
Lane 3:
Jurkat cell lysate at 20 µg
Predicted band size: 62 kDa
Observed band size: 75 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human COIL (Coilin) knockout HeLa cell lysate (AB257251)
Homozygous : 10 bp deletion in exon 1
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This protein facilitates the organization and biogenesis of nuclear bodies associated with RNA processing and small nuclear ribonucleoproteins (snRNPs) assembly. Coilin operates as part of a larger complex involving several other proteins that interact within Cajal bodies. These nuclear bodies serve as sites where components necessary for RNA processing are concentrated. Coilin aids in the trafficking and modification of snRNP elements which are essential for pre-mRNA splicing.
Pathways
Coilin affects RNA metabolism and processing notably within the spliceosome assembly pathway and in snRNP biogenesis. The pathways include interactions with proteins such as SMN (Survival of Motor Neuron) protein which links to spinal muscular atrophy-related processes. Additionally Coilin contributes to the broader framework of nucleic acid processing systems impacting the outcome of RNA transcription and modification through its regulatory roles.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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