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AB258824

Human COL4A4 (Collagen alpha-4(IV) chain) knockout HeLa cell lysate

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COL4A4 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon29 and 2 bp insertion in exon29.

View Alternative Names

CO4A4_HUMAN, COL4A4, Collagen alpha-4(IV) chain

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Sanger Sequencing - Human COL4A4 (Collagen alpha-4(IV) chain) knockout HeLa cell lysate (AB258824)
  • Sanger seq

Unknown

Sanger Sequencing - Human COL4A4 (Collagen alpha-4(IV) chain) knockout HeLa cell lysate (AB258824)

Allele-2 : 2 bp insertion in exon29

Sanger Sequencing - Human COL4A4 (Collagen alpha-4(IV) chain) knockout HeLa cell lysate (AB258824)
  • Sanger seq

Unknown

Sanger Sequencing - Human COL4A4 (Collagen alpha-4(IV) chain) knockout HeLa cell lysate (AB258824)

Allele-1 : 1 bp deletion in exon29

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon29 and 2 bp insertion in exon29.

Disease

Adenocarcinoma

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
COL4A4
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Collagen alpha-4(IV) chain often referred to as COL4A4 is a component of type IV collagen. This protein plays a mechanical role by forming a network in the basement membranes which are thin sheets of specialized extracellular matrix materials. It possesses a significant molecular mass of approximately 185 kDa. COL4A4 is mainly expressed in tissues such as the kidney cochlea and ocular structures where it integrates with other collagen chains to provide structural support and stability.
Biological function summary

The Collagen alpha-4(IV) chain contributes to the integrity and function of basement membranes. It forms a heterotrimeric complex with other alpha chains specifically with COL4A3 and COL4A5 which together are essential for maintaining the filtration barrier in the glomeruli of kidneys. This complex also plays roles in hearing and maintaining visual function. The structural rigidity and filtration properties provided by these collagen chains are vital for the proper functioning of these tissues.

Pathways

The Collagen alpha-4(IV) chain is significantly involved in the basement membrane organization pathway. It interacts closely with other collagens and cell surface receptors to mediate cell adhesion migration and signaling processes. Within the same pathway proteins such as laminins and integrins work similarly to ensure the stability and communication between cells and the extracellular matrix. Through these interactions the COL4A4 chain ensures proper tissue organization and cellular responses to environmental changes.

COL4A4 mutations relate to Alport syndrome and thin basement membrane nephropathy. Alport syndrome is a genetic disorder characterized by kidney disease hearing loss and ocular abnormalities resulting from defects in the collagen type IV structure. In this context the COL4A4 chain interacts with COL4A3 and COL4A5 and mutations in any of these chains disrupt the collagen network causing the disease symptoms. Understanding these associations aids in diagnosing and devising potential therapeutic strategies for these disorders.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

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