Human COL4A6 (Collagen IV alpha 6) knockout HeLa cell lysate
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COL4A6 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon24 and 2 bp deletion in exon24 and 4 bp deletion in exon24.
View Alternative Names
COL4A6, Collagen Alpha 6(IV), Collagen IV alpha 6 polypeptide, Collagen alpha 6(IV) chain, Collagen of basement membrane alpha 6, Collagen type IV alpha 6, MGC88184, Type IV alpha 6 collagen, dJ889N15.4
- Sanger seq
Unknown
Sanger Sequencing - Human COL4A6 (Collagen IV alpha 6) knockout HeLa cell lysate (AB258373)
Allele-1 : 4 bp deletion in exon24
- Sanger seq
Unknown
Sanger Sequencing - Human COL4A6 (Collagen IV alpha 6) knockout HeLa cell lysate (AB258373)
Allele-2 : 2 bp deletion in exon24
- Sanger seq
Unknown
Sanger Sequencing - Human COL4A6 (Collagen IV alpha 6) knockout HeLa cell lysate (AB258373)
Allele-3 : 1 bp insertion in exon24
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
As part of the collagen IV network COL4A6 contributes to the formation of the heterotrimeric type IV collagen molecules. These trimers are composed of three alpha chains that associate to form a complex structure necessary for providing mechanical support and filtration functions in the basement membrane. The involvement of COL4A6 in these processes highlights its important role in tissue morphogenesis and maintenance.
Pathways
The function of COL4A6 integrates into major signaling cascades like the integrin-mediated signaling pathway and the extracellular matrix (ECM) remodeling pathway. Within these pathways COL4A6 collaborates with other collagen IV proteins such as COL4A1 and COL4A5 to interact with integrins. These interactions are critical for signal transduction processes that regulate cell adhesion migration and differentiation thereby maintaining tissue architecture.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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