Human COLGALT1 (GLT25D1) knockout HEK-293T cell lysate
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COLGALT1 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 5 bp deletion in exon2.
View Alternative Names
FLJ22329, Glycosyltransferase 25 domain containing 1, Hydroxylysine galactosyltransferase 1, Procollagen galactosyltransferase 1
- Sanger seq
Unknown
Sanger Sequencing - Human COLGALT1 (GLT25D1) knockout HEK-293T cell lysate (AB258825)
Homozygous : 5 bp deletion in exon2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
GLT25D1 plays a significant role in the post-translational modification of collagen. It participates in forming the galactosylhydroxylysine residues which are important for collagen's structural integrity and function. The enzyme may function as part of a multiprotein complex which ensures efficient modification of the collagen fibers during their biosynthesis. Through these modifications the enzyme affects the fibrillar assembly that is essential for connective tissue strength and elasticity.
Pathways
The role of GLT25D1 is prominent in the collagen synthesis pathway. This pathway involves not only the synthesis of collagen polypeptides but also their modification folding and secretion. The GLT25D1 enzyme works in conjunction with other glycosyltransferases like galactosyltransferase 2 (GLT25D2) to ensure collagen molecules are properly glycosylated and transported through the secretory pathway. These enzymes together regulate the mechanical properties of the extracellular matrix which is important for tissue function.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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