Human COQ9 knockout HEK-293T cell lysate
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COQ9 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon1.
View Alternative Names
C16orf49, COQ9_HUMAN, Chromosome 16 open reading frame 49, Coenzyme Q9 homolog (S. cerevisiae), Coenzyme Q9 homolog (yeast), HSPC326, PSEC0129, Ubiquinone biosynthesis protein COQ9, Ubiquinone biosynthesis protein COQ9, mitochondrial, mitochondrial
- Sanger seq
Unknown
Sanger Sequencing - Human COQ9 knockout HEK-293T cell lysate (AB258376)
Homozygous : Insertion of the selection cassette in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
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It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
COQ9 operates within a multi-subunit complex called the CoQ biosynthesis complex. This complex supports the synthesis of coenzyme Q10 a molecule involved in cellular respiration. COQ9's function ensures the stability and efficiency of the CoQ biosynthesis process which is necessary for mitochondrial health and energy production. Its role is supported by interactions with other proteins in the complex fostering effective biosynthesis of this quinone compound.
Pathways
The COQ9 protein is an important player in the ubiquinone biosynthesis pathway and indirectly influences the electron transport chain. COQ9 associates closely with other COQ proteins such as COQ8 which collectively drive the coenzyme Q production essential for the electron transport chain's proper function. Defects in this pathway can disrupt cellular energy metabolism highlighting COQ9's significance.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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