COQ9 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon1.
Images
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon1.
Alternative names
C16orf49, COQ9_HUMAN, Chromosome 16 open reading frame 49, Coenzyme Q9 homolog (S. cerevisiae), Coenzyme Q9 homolog (yeast), HSPC326, PSEC0129, Ubiquinone biosynthesis protein COQ9, Ubiquinone biosynthesis protein COQ9, mitochondrial, mitochondrial
What's included?
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COQ9 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon1.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon1.
- Concentration
Properties
- Gene name
- COQ9
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
COQ9 also known as coenzyme Q9 homolog is a protein integral to coenzyme Q (ubiquinone) biosynthesis. It has a molecular mass of approximately 30 kDa. COQ9 expresses mainly in mitochondria the energy centers of cells. This localization aligns with its role in the mitochondrial respiratory chain where it assists in ubiquinone production essential for electron transport and ATP generation.
- Biological function summary
COQ9 operates within a multi-subunit complex called the CoQ biosynthesis complex. This complex supports the synthesis of coenzyme Q10 a molecule involved in cellular respiration. COQ9's function ensures the stability and efficiency of the CoQ biosynthesis process which is necessary for mitochondrial health and energy production. Its role is supported by interactions with other proteins in the complex fostering effective biosynthesis of this quinone compound.
- Pathways
The COQ9 protein is an important player in the ubiquinone biosynthesis pathway and indirectly influences the electron transport chain. COQ9 associates closely with other COQ proteins such as COQ8 which collectively drive the coenzyme Q production essential for the electron transport chain's proper function. Defects in this pathway can disrupt cellular energy metabolism highlighting COQ9's significance.
- Associated diseases and disorders
Dysfunctions in COQ9 relate to conditions like primary coenzyme Q10 deficiency and mitochondrial encephalomyopathies. These disorders stem from inadequate coenzyme Q10 production leading to impaired mitochondrial function. COQ9 deficiencies can involve COQ2 mutations as both proteins participate in coenzyme Q10 biosynthesis further linking COQ9 to these pathological states.
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1 product image
Sanger Sequencing - Human COQ9 knockout HEK-293T cell lysate (ab258376)
Homozygous: Insertion of the selection cassette in exon1
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- Visit the general protocols
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